Department of Anesthesiology, Akdeniz University Medical Faculty, 07070, Antalya, Turkey.
Department of Pathology, Akdeniz University Medical Faculty, 07070, Antalya, Turkey.
Food Chem Toxicol. 2018 Aug;118:795-804. doi: 10.1016/j.fct.2018.06.038. Epub 2018 Jun 20.
The aim of this study was to investigate whether thymoquinone (TQ) could alleviate diclofenac (DCLF)-induced gastrointestinal and renal toxicity in rats. Diclofenac was administered via intramuscular injection twice daily for 5 days and TQ was given by gavage for the same period. Hematological and biochemical profiles were measured with autoanalyzers while reactive oxygen/nitrogen species (ROS/RNS) generation and total antioxidant capacity (TAC) were assayed by standard kits. Tissue injuries were evaluated by microscopy and histopathological scoring. Diclofenac treatment caused kidney and liver function test abnormalities, reduced hematocrit and hemoglobin levels but increased WBC and platelet counts. Histopathological findings showed renal tubular damage, gastrointestinal lesions and increased fibrosis in DCLF treated rats. Thymoquinone administration, along with DCLF treatment, attenuated hematological test abnormalities and DCLF induced renal functional impairment as evident by significantly restored serum creatinine and blood urea nitrogen levels. Similarly, TQ treatment significantly alleviated liver function test abnormalities and decreased tissue injury in the stomach and duodenum. Diclofenac treatment caused increased ROS/RNS formation and decreased TAC in the kidney, stomach and duodenal tissue. Thymoquinone administration increased gastrointestinal and renal TAC in DCLF treated rats. These results indicate that TQ could ameliorate gastrointestinal and renal toxicity induced by high dose DCLF treatment.
本研究旨在探讨姜黄素（TQ）是否能减轻双氯芬酸（DCLF）诱导的大鼠胃肠道和肾脏毒性。双氯芬酸通过肌肉注射每天两次给药 5 天，同时给予 TQ 灌胃。用自动分析仪测量血液学和生化参数，用标准试剂盒测定活性氧/氮物种（ROS/RNS）生成和总抗氧化能力（TAC）。通过显微镜和组织病理学评分评估组织损伤。双氯芬酸治疗导致肾功能和肝功能试验异常，降低红细胞压积和血红蛋白水平，但增加白细胞和血小板计数。组织病理学发现肾小管损伤、胃肠道病变和 DCLF 治疗大鼠的纤维化增加。姜黄素给药与 DCLF 治疗同时进行，可减轻血液学试验异常和 DCLF 引起的肾功能损害，表现为血清肌酐和血尿素氮水平显著恢复。同样，TQ 治疗可显著减轻肝功能试验异常和胃、十二指肠组织损伤。双氯芬酸治疗导致肾脏、胃和十二指肠组织中 ROS/RNS 形成增加和 TAC 降低。TQ 给药增加了 DCLF 治疗大鼠的胃肠道和肾脏 TAC。这些结果表明，TQ 可以改善高剂量 DCLF 治疗引起的胃肠道和肾脏毒性。
