Ben-Shachar D, Finberg J P, Youdim M B
J Neurochem. 1985 Oct;45(4):999-1005. doi: 10.1111/j.1471-4159.1985.tb05514.x.
Nutritional iron deficiency induced in rats causes a selective reduction of [3H]spiperone binding in caudate nucleus. This effect can be reversed by iron supplementation in vivo. The possibility that iron may be involved in the dopamine D2 receptor was investigated by examining the effect of various iron and noniron chelators on the binding of [3H]spiperone in rat caudate nucleus. Iron chelators 1,10-phenanthroline, 2,4,6-tripyridyl-s-triazine, alpha, alpha'-dipyridyl, and desferrioxamine mesylate inhibited the binding of [3H]spiperone. The inhibition by 1,10-phenanthroline was noncompetitive and reversible. In the presence of FeCl2 or FeCl3, the inhibitory effect of 1,10-phenanthroline was potentiated. Iron salts or chelators were without effect on the binding of [3H]dihydroalprenolol to beta-adrenoreceptors in caudate nucleus; thus the action of iron chelators on the dopamine D2 receptor tends to be selective. Incubation of caudate nucleus membrane prepared from iron-deficient rats with FeCl2 or FeCl3 did not reverse the diminished binding of [3H]spiperone. The present study indicates that if iron is involved in the physiological regulation of dopamine D2 agonist-antagonist binding sites, it is more complex than hitherto considered.
给大鼠诱导造成的营养性缺铁会导致其尾状核中[3H]螺哌隆结合选择性减少。这种效应可通过体内补充铁来逆转。通过研究各种铁螯合剂和非铁螯合剂对大鼠尾状核中[3H]螺哌隆结合的影响，来探究铁是否可能参与多巴胺D2受体的作用。铁螯合剂1,10 - 菲啰啉、2,4,6 - 三吡啶基 - s - 三嗪、α,α'-联吡啶和甲磺酸去铁胺抑制了[3H]螺哌隆的结合。1,10 - 菲啰啉的抑制作用是非竞争性且可逆的。在氯化亚铁或氯化铁存在的情况下，1,10 - 菲啰啉的抑制作用会增强。铁盐或螯合剂对尾状核中[3H]二氢阿普洛尔与β - 肾上腺素受体的结合没有影响；因此，铁螯合剂对多巴胺D2受体的作用往往具有选择性。用氯化亚铁或氯化铁孵育缺铁大鼠制备的尾状核膜，并不能逆转[3H]螺哌隆结合减少的情况。本研究表明，如果铁参与多巴胺D2激动剂 - 拮抗剂结合位点的生理调节，其机制比迄今所认为的更为复杂。
