秀丽隐杆线虫在高通量筛选抗感染化合物中的应用。
Caenorhabditis elegans in high-throughput screens for anti-infective compounds.
机构信息
Program in Innate Immunity, Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01655, United States.
Program in Innate Immunity, Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01655, United States.
出版信息
Curr Opin Immunol. 2018 Oct;54:59-65. doi: 10.1016/j.coi.2018.06.003. Epub 2018 Jun 20.
Abstract
New classes of antimicrobials that are effective therapies for infections with multi-drug resistant pathogens are urgently needed. The nematode Caenorhabditis elegans has been incorporated into small molecule screening platforms to identify anti-infective compounds that provide protection of a host during infection. The use of a live animal in these screening systems offers several advantages, including the ability to identify molecules that boost innate immune responses in a manner advantageous to host survival and compounds that disrupt bacterial virulence mechanisms. In addition, new classes of antimicrobials that target the pathogen have been uncovered, as well as interesting chemical probes that can be used to dissect new mechanisms of host-pathogen interactions.
摘要
急需开发新的抗生素类别，以有效治疗对抗生素具有多重耐药性的病原体感染。秀丽隐杆线虫已被纳入小分子筛选平台，用于鉴定在感染过程中为宿主提供保护的抗感染化合物。在这些筛选系统中使用活体动物具有多种优势，包括能够识别以有利于宿主生存的方式增强先天免疫反应的分子，以及破坏细菌毒力机制的化合物。此外，还发现了针对病原体的新类别的抗生素，以及可用于剖析宿主-病原体相互作用新机制的有趣化学探针。
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