Desanlis Ines, Felstead Hannah L, Edwards Dylan R, Wheeler Grant N
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
Gene Expr Patterns. 2018 Sep;29:72-81. doi: 10.1016/j.gep.2018.06.001. Epub 2018 Jun 21.
Extracellular matrix (ECM) remodeling by metalloproteinases is crucial during development. The ADAMTS (A Disintegrin and Metalloproteinase with Thrombospondin type I motifs) enzymes are secreted, multi-domain matrix-associated zinc metalloendopeptidases that have diverse roles in tissue morphogenesis and patho-physiological remodeling. The human family includes 19 members. In this study we identified the 19 members of the ADAMTS family in Xenopus laevis and Xenopus tropicalis. Gene identification and a phylogenetic study revealed strong conservation of the ADAMTS family and contributed to a better annotation of the Xenopus genomes. Expression of the entire ADAMTS family was studied from early stages to tadpole stages of Xenopus, and detailed analysis of ADAMTS9 revealed expression in many structures during organogenesis such as neural crest (NC) derivative tissues, the pronephros and the pancreas. Versican, a matrix component substrate of ADAMTS9 shows a similar expression pattern suggesting a role of ADAMTS9 in the remodeling of the ECM in these structures by degradation of versican.
金属蛋白酶介导的细胞外基质（ECM）重塑在发育过程中至关重要。ADAMTS（具有I型血小板反应蛋白基序的解聚素和金属蛋白酶）酶是分泌型、多结构域的基质相关锌金属内肽酶，在组织形态发生和病理生理重塑中具有多种作用。人类家族包括19个成员。在本研究中，我们在非洲爪蟾和热带爪蟾中鉴定出了ADAMTS家族的19个成员。基因鉴定和系统发育研究揭示了ADAMTS家族的高度保守性，并有助于更好地注释爪蟾基因组。我们研究了非洲爪蟾从早期到蝌蚪期整个ADAMTS家族的表达情况，对ADAMTS9的详细分析显示其在器官发生过程中的许多结构中表达，如神经嵴（NC）衍生组织、前肾和胰腺。ADAMTS9的基质成分底物多功能蛋白聚糖显示出相似的表达模式，这表明ADAMTS9通过降解多功能蛋白聚糖在这些结构的ECM重塑中发挥作用。
