Department of Bioresource Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
Department of Bioresource Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan; The United Graduate School of Agricultural Science, Gifu University (Shizuoka University), Yanagido, Gifu 501-1193, Japan.
Plant Physiol Biochem. 2018 Aug;129:349-356. doi: 10.1016/j.plaphy.2018.06.015. Epub 2018 Jun 21.
In the present study, carotenoid metabolism was investigated in the fruits of a novel citrus cultivar, 'Seinannohikari' (Citrus spp.). During the maturation, β,β-xanthophylls were accumulated rapidly with β-cryptoxanthin being the dominant carotenoid compound in the flavedo and juice sacs of 'Seinannohikari'. In the juice sacs of mature fruits, 'Seinannohikari' accumulated high amount of carotenoids, especially β-cryptoxanthin. The content of β-cryptoxanthin in the juice sacs of 'Seinannohikari' was approximately 2.5 times of that in 'Miyagawa-wase' (Citrus unshiu), which is one of its parental cultivars, at the mature stage. Gene expression results showed that the massive accumulation of β-cryptoxanthin might be attributed to the higher expression of carotenoid biosynthetic genes (CitPSY, CitPDS, CitZDS, CitLCYb2, CitHYb, and CitZEP), and lower expression of carotenoid catabolic genes (CitNCED2 and CitNCED3) in the juice sacs of 'Seinannohikari'.
本研究调查了新型柑橘品种“ Seinannohikari”（柑橘属）果实中的类胡萝卜素代谢。在成熟过程中，β，β-叶黄素迅速积累，β-隐黄质是“ Seinannohikari”果皮和汁囊的主要类胡萝卜素化合物。在成熟果实的汁囊中，“ Seinannohikari”积累了大量的类胡萝卜素，特别是β-隐黄质。“ Seinannohikari”汁囊中的β-隐黄质含量在成熟阶段约为其亲本品种“宫川早生”（Citrus unshiu）的 2.5 倍。基因表达结果表明，β-隐黄质的大量积累可能归因于汁囊中类胡萝卜素生物合成基因（CitPSY、CitPDS、CitZDS、CitLCYb2、CitHYb 和 CitZEP）的高表达，以及类胡萝卜素降解基因（CitNCED2 和 CitNCED3）的低表达。
