Tenen D G, Haines L L, Hansen U M, Martin R G, Livingston D M
J Virol. 1985 Oct;56(1):293-7. doi: 10.1128/JVI.56.1.293-297.1985.
Heteroduplex DNA molecules were formed by annealing an intact simian virus replication origin-containing fragment to a mutant derivative lacking the indigenous wild-type 27-base-pair (bp) inverted repeat within this structure and containing a nonhomologous 26-bp inverted repeat sequence in its place. Results of restriction enzyme and S1 endonuclease cleavage analyses strongly suggested that a 13-bp stem-loop structure formed at the site of nonhomology between these two DNAs. This structure lies within the boundary of simian virus 40 T-antigen-binding site 2, and its presence inhibited T-antigen binding to that sequence but not to an adjacent higher-affinity binding site (site 1). Therefore, the conformation of sequences within an otherwise intact T-antigen-binding site can have major effects upon T-antigen binding there.
异源双链DNA分子是通过将一个完整的含有猿猴病毒复制起点的片段与一个突变衍生物退火形成的，该突变衍生物在此结构中缺乏原生野生型27碱基对（bp）的反向重复序列，取而代之的是一个非同源的26 bp反向重复序列。限制性内切酶和S1核酸内切酶切割分析结果强烈表明，在这两个DNA的非同源位点形成了一个13 bp的茎环结构。该结构位于猿猴病毒40 T抗原结合位点2的边界内，其存在抑制了T抗原与该序列的结合，但不影响与相邻高亲和力结合位点（位点1）的结合。因此，在其他方面完整的T抗原结合位点内序列的构象可对T抗原在该处的结合产生重大影响。
