Ryder K, Silver S, DeLucia A L, Fanning E, Tegtmeyer P
Cell. 1986 Mar 14;44(5):719-25. doi: 10.1016/0092-8674(86)90838-x.
Seventeen base pairs of DNA from SV40 origin region I encode a tripartite binding site for a dimeric mass of SV40 large T antigen. Two binding components are the directly repeated pentanucleotide sequences 5'-GAGGC-3'/5'-GCCTC-3'. The third component is the asymmetric sequence 5'-TTTTTTG-3'/5'-CAAAAAA-3' that separates the pentanucleotides. Nucleotide-specific features of this spacer element stabilize binding to the adjacent pentanucleotides. We report here that the spacer sequence determines a DNA conformation that correlates with high affinity binding of T antigen. The nature of the spacer sequence suggests that the DNA is bent. We propose that binding of T antigen to region I proceeds through monomer-pentanucleotide interactions and either protein-protein or protein-spacer interactions directed by the spacer-encoded structure.
来自SV40起始区域I的17个DNA碱基对编码一个用于SV40大T抗原二聚体的三联体结合位点。两个结合成分是直接重复的五核苷酸序列5'-GAGGC-3'/5'-GCCTC-3'。第三个成分是将五核苷酸分开的不对称序列5'-TTTTTTG-3'/5'-CAAAAAA-3'。这个间隔元件的核苷酸特异性特征稳定了与相邻五核苷酸的结合。我们在此报告，间隔序列决定了一种与T抗原高亲和力结合相关的DNA构象。间隔序列的性质表明DNA是弯曲的。我们提出，T抗原与区域I的结合是通过单体 - 五核苷酸相互作用以及由间隔编码结构指导的蛋白质 - 蛋白质或蛋白质 - 间隔相互作用进行的。
