Półrola Paweł, Wilk-Franczuk Magdalena, Wilczyński Jacek, Nowak-Starz Grażyna, Góral-Półrola Jolanta, Chruściński Grzegorz, Bonisławska Iwona, Pedrycz Agnieszka, Żychowska Małgorzata
Faculty of Medicine and Health Sciences, Jan Kochanowski University, Kielce, Poland.
Faculty of Health Sciences, Medical College, Jagiellonian University, Kraków, Poland.
Ann Agric Environ Med. 2018 Jun 20;25(2):329-333. doi: 10.26444/aaem/85208. Epub 2018 Mar 26.
Some studies have shown the influence of Qigong on gene expression in different cells, but there is little data associated with the influence of this kind of therapy on genes expression in pheripheral monocellucar blood cells.
The aim of this study was to evaluate changes in the expression of genes associated with cellular stress response in peripheral mononuclear blood cells (PMBC) in healthy women.
The experiment took place at the Japanese Martial Arts Centre "DOJO" in Stara Wieś, Poland, conducted over the course of a 4-day qigong training session. To evaluate the genes effect of this training, blood samples were taken before and after the training period. This experiment involved 20 healthy women (aged 56.2±9.01, body height 164.8±6.5 and mass 65.5±8.2). To determine the expression of HSF-1, HSPA1A, NF-kB, IL10 and CCL2 mRNA, 3 ml of venous blood was collected. The blood samples were placed in tubes allowing for separation (BD Vacutainer CPT ) before and after the 4 days of qigong training. Isolated PMBC were used to determine gene expression using real-time qRT-PCR (quantitative reverse transcription polymerase chain reaction).
Significant decreases in NF-kB and CCL2 mRNA and increases in IL10, HSF1 and HSPA1A m-RNA were detected after 4 days of qigong training. The obtained findings suggest that qigong caused a reduction in the inflammatory and intensified anti-inflammatory gene expression, as well as a higher expression of HSF-1 and HSPA1A.
The adaptive response to qigong training was similar to the adaptive response to physical activity and was detected through gene expression in PMBC. Furthermore, this kind of training is especially indicated for women because of their higher susceptibility to psychosocial stress when compared to men.
一些研究显示了气功对不同细胞中基因表达的影响，但关于这种疗法对外周单个核血细胞基因表达影响的数据却很少。
本研究旨在评估健康女性外周单个核血细胞（PMBC）中与细胞应激反应相关基因表达的变化。
实验在波兰旧维谢的日本武术中心“道场”进行，为期4天的气功训练期间展开。为评估此次训练对基因的影响，在训练前后采集血样。该实验纳入了20名健康女性（年龄56.2±9.01岁，身高164.8±6.5厘米，体重65.5±8.2千克）。为测定HSF-1、HSPA1A、NF-κB、IL10和CCL2 mRNA的表达，采集了3毫升静脉血。在4天的气功训练前后，将血样置于可分离的试管中（BD Vacutainer CPT）。分离得到的PMBC用于通过实时定量逆转录聚合酶链反应（qRT-PCR）测定基因表达。
气功训练4天后，检测到NF-κB和CCL2 mRNA显著下降，IL10、HSF1和HSPA1A mRNA增加。所得结果表明，气功导致炎症相关基因表达降低，抗炎基因表达增强，以及HSF-1和HSPA1A表达升高。
对气功训练的适应性反应与对体育活动的适应性反应相似，可通过PMBC中的基因表达检测到。此外，由于女性比男性更容易受到心理社会压力的影响，这种训练对女性尤为适用。
