Ueki H, Abe R, Kimura M, Kasai M
Gan No Rinsho. 1985 Jul;31(8):929-32.
Flow cytometric DNA analyses of single cell nuclei were performed on nuclear suspensions prepared from tumors of primary breast carcinomas in 40 patients operated on in our hospital. Fluorescence intensities of propidium-iodide stained cell nuclei were analyzed in a SHOWA DENKO SC-20 cell sorter. The DNA index and the percentage of S-phase cells and DNA aneuploidy were calculated from each DNA histogram. There was a tendency for more advanced nodal tumor involvement to have a higher DNA index, increasing DNA aneuploidy proportions and significantly larger percent of S-phase cells.
对在我院接受手术的40例原发性乳腺癌患者肿瘤制备的核悬液进行了单细胞细胞核的流式细胞术DNA分析。在昭和电工SC - 20细胞分选仪中分析碘化丙啶染色细胞核的荧光强度。从每个DNA直方图计算DNA指数、S期细胞百分比和DNA非整倍体率。淋巴结肿瘤受累程度越高，DNA指数越高、DNA非整倍体比例增加以及S期细胞百分比显著越大，存在这种趋势。
