Weiss H, Kunde D, Streller B
Arch Geschwulstforsch. 1986;56(5):373-9.
DNA distributions were recorded flow cytometrically in 19 normal, 23 precancerous tissues with epithelial proliferation and cell atypia (P1), 7 carcinomata lobularia in situ and intraductale carcinomas (P2) and in 44 invasive carcinomas of the human breast. The results were compared to histological findings, tumor staging and grading, lymph node involvement and age of the patients. DNA diploidy was found in all normal, 21 P1 tissues, 6 P2 tissues and 12 carcinomas; DNA aneuploidy in 2 P1 tissues, 1 P2 tissue and 32 carcinomas. Lobular carcinomas were preferentially involved in the DNA diploid group, duct carcinomas in the DNA aneuploid group. Cell cycle distribution analysis resulted in significantly higher percentages of cells in S- and G2M phase in DNA aneuploid carcinomas compared to normal and precancerous (P1, P2) tissues. The percentage of cells in G2M phase was significantly higher in DNA aneuploid carcinomas originating from patients younger than 50 years compared to patients older than 50 years. Correlations between the DNA-index, cell cycle distribution, lymph node involvement, tumour staging or histological type were not evident.
采用流式细胞术记录了19例正常乳腺组织、23例伴有上皮增生和细胞异型性的癌前组织（P1）、7例小叶原位癌和导管内癌（P2）以及44例人乳腺浸润性癌的DNA分布情况。将结果与组织学检查结果、肿瘤分期和分级、淋巴结受累情况以及患者年龄进行了比较。在所有正常组织、21例P1组织、6例P2组织和12例癌组织中发现了DNA二倍体；在2例P1组织、1例P2组织和32例癌组织中发现了DNA非整倍体。小叶癌优先出现在DNA二倍体组中，导管癌则出现在DNA非整倍体组中。与正常组织和癌前组织（P1、P2）相比，DNA非整倍体癌组织中处于S期和G2M期的细胞百分比显著更高。与年龄大于50岁的患者相比，年龄小于50岁的患者所患DNA非整倍体癌组织中处于G2M期的细胞百分比显著更高。DNA指数、细胞周期分布、淋巴结受累情况、肿瘤分期或组织学类型之间均无明显相关性。
