Bedrossian C W, Raber M, Barlogie B
Anal Quant Cytol. 1981 Jun;3(2):112-6.
Using flow cytometry for DNA analysis, ploidy and proliferative activity were measured in 43 cases of primary resectable breast cancer, 24 of which were also assayed for estrogen receptors. Tumors were studied histopathologically by multiple samples and cytopathologically by means of imprint smears and pepsinized cell suspensions. Cytologic preservation was far superior in imprint smears than in cell suspensions or histologic sections. As determined by the DNA index (tumor G1/G0:normal G1/G0), 72% of the tumors were aneuploid, with the majority falling between diploid and tetraploid. There was no correlation of ploidy with tumor cell proliferation expressed as percentages in the S-phase. A high percentage of S-phase cells was associated with estrogen receptor negativity and tended to correlate with cytologic atypia but showed no correlation with UICC stage, menopausal status, tumor size, lymph node involvement or histologic degree of malignancy. In contrast, ploidy showed no correlation with any of the above parameters but served as a reliable marker for individual tumors since it did not change with time in multiple biopsies of the primary site.
采用流式细胞术进行DNA分析，对43例原发性可切除乳腺癌患者测量其倍性和增殖活性，其中24例还检测了雌激素受体。通过多个样本对肿瘤进行组织病理学研究，并通过印片涂片和胃蛋白酶消化细胞悬液进行细胞病理学研究。细胞学保存效果在印片涂片中远优于细胞悬液或组织切片。根据DNA指数（肿瘤G1/G0：正常G1/G0）测定，72%的肿瘤为非整倍体，大多数介于二倍体和四倍体之间。倍性与以S期百分比表示的肿瘤细胞增殖无相关性。高比例的S期细胞与雌激素受体阴性相关，且倾向于与细胞学异型性相关，但与国际抗癌联盟（UICC）分期、绝经状态、肿瘤大小、淋巴结受累情况或组织学恶性程度无关。相比之下，倍性与上述任何参数均无相关性，但可作为单个肿瘤的可靠标志物，因为在原发部位的多次活检中其不会随时间变化。
