Bradic Jovana, Dragojlovic Ruzicic Radica, Jeremic Jovana, Petkovic Anica, Stojic Isidora, Nikolic Tamara, Zivkovic Vladimir, Srejovic Ivan, Radovanovic Dragan, Jakovljevic Vladimir Lj
University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia.
Gen Physiol Biophys. 2018 May;37(3):285-297. doi: 10.4149/gpb_2017053.
Given the fact that oxidative stress response induced by training/detraining has still not been clarified and may be influenced by gender, the aim of our investigation was to compare the effects of swimming training and detraining on oxidative and antioxidative parameters in rats, with a special focus on sex differences. Wistar albino rats (n = 64) were divided into 4 groups: control, trained group, groups exposed to 2 and 4 weeks of detraining. Each group included two subgroups: males and females. After sacrificing, hearts were isolated and retrogradely perfused according to Langendorff technique. Levels of superoxide anion radical, hydrogen peroxide, nitrites and thiobarbituric acid reactive substances were measured in plasma and coronary venous effluent, while reduced glutathione, activities of superoxide dismutase and catalase were measured in erythrocytes. Our results indicate that swimming training doesn't promote oxidative damage, nor act protectively within the heart. However, 2 and 4 weeks of detraining led to a partial lost in exercise-induced adaptation. It seems that moderate-intensity physical exercise of sufficient duration leads to beneficial adaptations, which may be partially lost during detraining period. Positive antioxidative effects of training remained longer in males. Findings of present study may help in elucidation of training and detraining effects on modulation of redox homeostasis, especially from aspect of gender differences.
鉴于训练/停训所诱导的氧化应激反应仍未明确，且可能受性别影响，我们研究的目的是比较游泳训练和停训对大鼠氧化和抗氧化参数的影响，特别关注性别差异。将64只Wistar白化大鼠分为4组：对照组、训练组、停训2周组和停训4周组。每组包括两个亚组：雄性和雌性。处死后，按照Langendorff技术分离心脏并进行逆行灌注。测定血浆和冠状静脉流出液中超氧阴离子自由基、过氧化氢、亚硝酸盐和硫代巴比妥酸反应性物质的水平，同时测定红细胞中还原型谷胱甘肽、超氧化物歧化酶和过氧化氢酶的活性。我们的结果表明，游泳训练既不促进氧化损伤，也不在心脏内发挥保护作用。然而，2周和4周的停训导致运动诱导的适应性部分丧失。似乎足够时长的中等强度体育锻炼会导致有益的适应性变化，这些变化在停训期间可能会部分丧失。训练的积极抗氧化作用在雄性中持续时间更长。本研究结果可能有助于阐明训练和停训对氧化还原稳态调节的影响，特别是从性别差异的角度。
