Butruk-Raszeja Beata A, Kuźmińska Aleksandra
Laboratory of Biomedical Engineering, Faculty of Chemical & Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Biotechniques. 2018 Jun;64(6):245-253. doi: 10.2144/btn-2018-0004.
The goal in the presented study was to develop a simple, fast and accurate method for measuring the surface density of a short peptide sequence bound to a polymeric substrate. We analyzed polyurethane samples chemically modified with acrylic acid and polyurethane-grafted peptide (GSGREDVGSG) and investigated the possibility of using the bicinchoninic acid (BCA) assay to determine surface density of the solid-supported peptide. We set the conditions (temperature, time) under which the test should be conducted. We also studied the interaction of the BCA reagent with polyurethane substrate and the effect of drying conditions as well as material type and form on the test response. We have proposed potential factors that might interfere with the BCA assay and chosen the proper control materials.
本研究的目标是开发一种简单、快速且准确的方法，用于测量与聚合物基质结合的短肽序列的表面密度。我们分析了用丙烯酸化学改性的聚氨酯样品以及聚氨酯接枝肽（GSGREDVGSG），并研究了使用二辛可宁酸（BCA）测定法来确定固相支持肽表面密度的可能性。我们设定了进行测试的条件（温度、时间）。我们还研究了BCA试剂与聚氨酯基质的相互作用以及干燥条件、材料类型和形态对测试响应的影响。我们提出了可能干扰BCA测定的潜在因素，并选择了合适的对照材料。
