稀释及复杂样品中蛋白质的定量分析：二辛可宁酸检测法的改良
Quantification of protein in dilute and complex samples: modification of the bicinchoninic acid assay.
作者信息
Schoel B, Welzel M, Kaufmann S H
机构信息
Department of Immunology, University of Ulm, Germany.
出版信息
J Biochem Biophys Methods. 1995 Jun;30(2-3):199-206. doi: 10.1016/0165-022x(95)00009-g.
The colorimetric assay using bicinchoninic acid (BCA) as test reagent is useful for quantitative protein determinations due to its high sensitivity, ease, and tolerance to various contaminations present in biological samples or added during purification. For removal of interfering substances, protein precipitations have been described. Yet, obstructions became apparent with diluted and complex samples. Therefore we tested different solvents for removal of such interfering contaminants from the protein precipitate, and 1 M HCl was identified as the useful washing agent. The protocol described allows simple and accurate microdetermination in microtiter plates of proteins from complex samples.
使用二辛可宁酸（BCA）作为测试试剂的比色测定法，因其高灵敏度、操作简便以及对生物样品中存在的或纯化过程中添加的各种污染物的耐受性，而可用于蛋白质的定量测定。为了去除干扰物质，人们已经描述了蛋白质沉淀法。然而，对于稀释的复杂样品，干扰问题变得明显。因此，我们测试了不同的溶剂，以从蛋白质沉淀中去除此类干扰污染物，结果确定1 M盐酸是一种有效的清洗剂。所描述的方案允许在微量滴定板中对复杂样品中的蛋白质进行简单而准确的微量测定。
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