关于DNA聚合酶α的保真度：α-硫代脱氧核苷三磷酸、锰离子和错配修复的影响
On the fidelity of DNA polymerase alpha: the influence of alpha-thio dNTPs, Mn2+ and mismatch repair.
作者信息
Rienitz A, Grosse F, Blöcker H, Frank R, Krauss G
出版信息
Nucleic Acids Res. 1985 Aug 12;13(15):5685-95. doi: 10.1093/nar/13.15.5685.
The phi X174am16 revertant system has been used to investigate the influence of alpha-thio-dNTPs and of Mn2+ on the fidelity of the 9S DNA polymerase alpha from calf thymus. Upon substituting dGTP by alpha-thio-dGTP during the in vitro replication, a nearly tenfold decrease in the frequency of G:G and G:T mispairs is observed. The formation of all other mispairs is not changed in the presence of the corresponding alpha-thio-dNTP. Mn2+ at concentrations of 0.5 mM does not influence the frequencies of the mispairs. The expression rate of errors formed during in vitro replication in the (-) strand has been determined for all mispairs detectable in the phi Xam16 system. The (-) strand expression of G:T, T:T and C:T mismatches is about 50%, whereas for A:G, G:G and C:A mismatches it is clearly below 50%. We conclude that the different base-base mismatches are repaired with different efficiencies.
噬菌体φX174am16回复系统已被用于研究α-硫代脱氧核苷三磷酸（alpha-thio-dNTPs）和锰离子（Mn2+）对小牛胸腺9S DNA聚合酶α保真度的影响。在体外复制过程中，用α-硫代脱氧鸟苷三磷酸（alpha-thio-dGTP）替代脱氧鸟苷三磷酸（dGTP）时，观察到G:G和G:T错配频率下降了近10倍。在相应的α-硫代脱氧核苷三磷酸存在的情况下，所有其他错配的形成没有改变。浓度为0.5 mM的Mn2+不影响错配频率。对于噬菌体φXam16系统中可检测到的所有错配，已测定了体外复制过程中在（-）链上形成错误的表达率。G:T、T:T和C:T错配的（-）链表达率约为50%，而对于A:G、G:G和C:A错配，其表达率明显低于50%。我们得出结论，不同的碱基错配以不同的效率被修复。
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