Grosse F, Krauss G
Biochemistry. 1981 Sep 15;20(19):5470-5. doi: 10.1021/bi00522a019.
a DNA polymerase alpha species from calf thymus has been purified 12 000-fold to near homogeneity. The enzyme sediments under high salt conditions in the preparative ultracentrifuge as a homogeneous band at 9S. The specific activity is 50 000-70 000 units/mg of protein. Polypeptides of 148 000, 59 000, and 48 000 daltons are detectable. The molecular weight as estimated from gradient gel electrophoresis is about 500 000. The 9S DNA polymerase is free from terminal nucleotidyl transferase activity and does not exhibit endonuclease or exonuclease activity. It is inhibited by low concentrations of salt, aphidicolin, and N-ethylmaleimide.
从小牛胸腺中纯化出一种DNA聚合酶α，纯化倍数达12000倍，接近均一。在高盐条件下，该酶在制备型超速离心机中以9S的均一带状形式沉降。比活性为50000 - 70000单位/毫克蛋白质。可检测到分子量为148000、59000和48000道尔顿的多肽。根据梯度凝胶电泳估计，其分子量约为500000。9S DNA聚合酶没有末端核苷酸转移酶活性，也不表现出内切酶或外切酶活性。它受到低浓度盐、阿非迪霉素和N - 乙基马来酰亚胺的抑制。
