Lapeyre B, Caizergues-Ferrer M, Bouche G, Amalric F
Nucleic Acids Res. 1985 Aug 26;13(16):5805-16. doi: 10.1093/nar/13.16.5805.
Nucleoline (100 kDa) is the major nucleolar protein in exponentially growing cells that behaves like a nucleolar organizer protein and plays a key role in rDNA transcription and prerRNA processing. We reported the isolation of 5 cDNA clones by probing a cDNA library, constructed in the expression vector lambda gt11, with a polyclonal serum raised against nucleoline. A new immunoassay, using hybrid proteins (beta gal-cDNA encoded protein) was developed to establish that the isolated cDNAs encoded parts of nucleoline. A further confirmation resulted from the sequence comparison between the cDNA encoded peptide and a 42 aa peptide isolated from rat nucleoline (1). The 5 cDNAs overlapped extensively and covered more than 90% of a full length cDNA. By probing a Northern blot with the 100 kDa cDNA, a 2650 nucleotide polyA+ RNA was detected that contained just enough information to code for nucleoline.
核仁素（100 kDa）是指数生长期细胞中的主要核仁蛋白，其行为类似于核仁组织区蛋白，在核糖体DNA（rDNA）转录和前体核糖体RNA（pre-rRNA）加工中起关键作用。我们报道了通过用针对核仁素的多克隆血清筛选构建于表达载体λgt11中的cDNA文库，分离出5个cDNA克隆。开发了一种使用杂交蛋白（β-半乳糖苷酶-cDNA编码蛋白）的新免疫测定法，以确定分离出的cDNA编码核仁素的部分序列。通过比较cDNA编码肽与从大鼠核仁素中分离出的42个氨基酸的肽段序列，进一步证实了这一点。这5个cDNA有广泛的重叠，覆盖了全长cDNA的90%以上。用100 kDa的cDNA探针检测Northern印迹，检测到一个2650个核苷酸的多聚腺苷酸加（polyA+）RNA，其包含的信息刚好足以编码核仁素。
