人肌肉特异性碳酸酐酶CAIII的cDNA克隆的分离

Isolation of a cDNA clone for the human muscle specific carbonic anhydrase, CAIII.

作者信息

Lloyd J C, Isenberg H, Hopkinson D A, Edwards Y H

出版信息

Ann Hum Genet. 1985 Jul;49(3):241-51. doi: 10.1111/j.1469-1809.1985.tb01698.x.

DOI:10.1111/j.1469-1809.1985.tb01698.x

Abstract

The molecular cloning of cDNA for the human muscle specific carbonic anhydrase CAIII is described. The recombinant was isolated from a human muscle cDNA library prepared in the expression vector lambda gt11, and was characterized by hybridization selection and immunoprecipitation. A comparison of insert cDNA and mRNA sizes suggests that the cDNA is full length and includes extensive untranslated sequences. Preliminary sequence data have confirmed the authenticity of this clone and Southern blotting of human and rodent DNA indicates that it will be a useful probe in the analysis of somatic cell hybrids.

摘要

本文描述了人类肌肉特异性碳酸酐酶CAIII的cDNA分子克隆。该重组体是从用表达载体λgt11构建的人类肌肉cDNA文库中分离得到的，并通过杂交筛选和免疫沉淀进行了鉴定。插入的cDNA与mRNA大小的比较表明，该cDNA是全长的，并且包含大量非翻译序列。初步的序列数据证实了该克隆的真实性，人类和啮齿动物DNA的Southern印迹分析表明，它将是分析体细胞杂种的有用探针。

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