Gal Arnon, Burchell Richard K, Worth Andrew J, Lopez-Villallobos Nicolas, Marshall Jonathan C, MacNeill Amy L
1 School of Veterinary Science, Massey University, Palmerston North, New Zealand.
2 Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand.
Vet Pathol. 2018 Nov;55(6):853-860. doi: 10.1177/0300985818780469. Epub 2018 Jun 25.
Bone marrow (BM) cytology and histopathology are complementary tools used to investigate hematological diseases. The purpose of this study was to determine if there are site-dependent differences in the diagnostic quality, myeloid to erythroid ratio (MER), and discordant findings in samples from different sites in the same dog. Eighteen apparently healthy dogs were used in the study. The sequence of sample acquisition was randomized according to a Latin square, and samples for BM cytology and histology were collected from both humeri and both ilial crests immediately after death. Board-certified clinical and anatomical pathologists read the cytology and histology, respectively. The data were analyzed using a mixed-effect model. The site of BM acquisition did not affect BM sample quality. The rate of discordant clinical findings between sites was 0.05 (95% confidence interval, 0.01-0.13). In general, by cytology, the MERs were slightly but significantly greater in samples from the ilial crests than from the humeri ( P = .01). The measured MER for histology was nearly twice that for cytology for all sites ( P < .001). In conclusion, there was a low-rate, site-dependent discordance in diagnostic findings in BM samples and differences in MER between the ilial crest and the humerus. A similar study is justified in sick dogs with hematological disease to determine the effect of sampling site on discordant findings between sites.
骨髓（BM）细胞学和组织病理学是用于研究血液系统疾病的互补工具。本研究的目的是确定同一只犬不同部位样本在诊断质量、髓红比（MER）及不一致结果方面是否存在部位依赖性差异。本研究使用了18只看似健康的犬。样本采集顺序根据拉丁方随机安排，在犬死后立即从双侧肱骨和双侧髂嵴采集用于BM细胞学和组织学检查的样本。经过认证的临床病理学家和解剖病理学家分别解读细胞学和组织学结果。数据采用混合效应模型进行分析。BM采集部位不影响BM样本质量。不同部位间临床结果不一致的发生率为0.05（95%置信区间，0.01 - 0.13）。一般来说，通过细胞学检查，髂嵴样本中的MER略高于肱骨样本，但差异有统计学意义（P = 0.01）。所有部位组织学测量的MER几乎是细胞学测量值的两倍（P < 0.001）。总之，BM样本诊断结果存在低发生率的部位依赖性不一致，且髂嵴与肱骨之间的MER存在差异。对患有血液系统疾病的病犬进行类似研究，以确定采样部位对不同部位间不一致结果的影响是合理的。
