Abrams-Ogg Anthony C G, Defarges Alice, Foster Robert A, Bienzle Dorothee
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Vet Clin Pathol. 2012 Jun;41(2):235-42. doi: 10.1111/j.1939-165X.2012.00422.x. Epub 2012 Apr 6.
Commonly used 11 or 13ga needles are relatively large for collection of bone marrow (BM) biopsies from small dogs.
The aims of this study were to assess ease of BM collection and quality of specimens obtained from small dogs using 13 and 15ga needles and to determine if specimen encasement would improve quality.
Humeral and iliac biopsies obtained from 17 Beagle dogs with a 15ga needle and a power driver were compared with humeral biopsies obtained with a 13ga Jamshidi needle. Ease of collection (scored as 1 [very difficult] to 5 [very easy] for 3 components of collection with scores summed), section quality (scored as 1 [poor] to 5 [good]), and lesions at collection sites were scored. Quality of additional humeral biopsies obtained with a 15ga needle and wrapped in tissue paper prior to fixation was assessed.
Use of a 15ga needle to obtain a humeral BM biopsy was significantly easier (mean score ± SD = 13.6 ± 1.7) than obtaining humeral BM using a 13ga needle (11.4 ± 1.6; P < .001) or obtaining iliac BM using a 15ga needle (10.9 ± 2.0, P < .001). Quality of humeral biopsies obtained with a 13ga needle (3.9 ± 1.2) was better than for biopsies of the humerus (1.9 ± 1.3, P < .001) or ilium (1.4 ± 0.6, P < .001) using a 15ga needle. Only sites sampled with a 13ga needle were identifiable grossly after the procedure. In most biopsies, cell density and cellularity were lower when a 15ga needle was used. Paper-wrapping of biopsies did not improve quality.
In small dogs, collection of humeral BM biopsies using a 15g needle is feasible and more easily accomplished than collection using a 13ga needle. Hematopoiesis may be underestimated in specimens collected using the smaller needle.
对于从小型犬采集骨髓活检样本而言，常用的11号或13号针头相对较大。
本研究旨在评估使用13号和15号针头从小型犬采集骨髓的难易程度以及所获样本的质量，并确定样本包裹是否会提高质量。
将使用15号针头和动力驱动器从17只比格犬获取的肱骨和髂骨活检样本，与使用13号Jamshidi针头获取的肱骨活检样本进行比较。对采集的难易程度（采集的3个组成部分按1[非常困难]至5[非常容易]评分，分数相加）、切片质量（按1[差]至5[好]评分）以及采集部位的病变进行评分。评估使用15号针头获取并在固定前用薄纸包裹的额外肱骨活检样本的质量。
使用15号针头获取肱骨骨髓活检样本明显比使用13号针头获取肱骨骨髓样本（11.4±1.6；P<.001）或使用15号针头获取髂骨骨髓样本（10.9±2.0，P<.001）更容易（平均评分±标准差=13.6±1.7）。使用13号针头获取的肱骨活检样本质量（3.9±1.2）优于使用15号针头获取的肱骨（1.9±1.3，P<.001）或髂骨（1.4±0.6，P<.001）活检样本。术后仅使用13号针头采样的部位在大体上可识别。在大多数活检样本中，使用15号针头时细胞密度和细胞成分较低。活检样本的薄纸包裹并未提高质量。
在小型犬中，使用15号针头采集肱骨骨髓活检样本是可行的，且比使用13号针头采集更容易。使用较小针头采集的样本中造血功能可能被低估。
