Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Universiti Kebangsaan Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Universiti Kebangsaan Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
BMC Complement Altern Med. 2018 Jun 26;18(1):197. doi: 10.1186/s12906-018-2250-5.
One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs).
Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05% OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05% OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3.
There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05).
The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation.
上呼吸道相关疾病的分子机制之一是上皮-间充质转化（EMT）。油橄榄（OE）具有抗炎特性，因此具有很大的潜力来预防 EMT。本研究旨在探讨 OE 对原代人鼻呼吸上皮细胞（RECs）中 EMT 的影响。
分离呼吸上皮细胞并分为四组：对照组（未处理）、用 0.05%OE 处理组（OE 组）、用 5ng/ml 转化生长因子β1（TGFβ1 组）诱导 EMT 组和用 5ng/ml TGFβ1+0.05%OE 处理组（TGFβ1+OE 组）。评估 OE 处理对 RECs 生长动力学、形态和蛋白表达的影响。免疫细胞化学分析用于从第 1 天到第 3 天定量测定 E-钙粘蛋白和波形蛋白的总表达百分比。
在形态、生长动力学和蛋白表达方面，未经处理的 RECs 与 OE 处理的 RECs 之间没有显著差异。TGFβ1 诱导导致 RECs 具有拉长的纺锤形、较慢的增殖率、较高的波形蛋白表达和较低的 E-钙粘蛋白表达，与对照组相比。与 TGFβ1 诱导的 RECs 相比，TGFβ1+OE 组的细胞具有相似的上皮形态，但增殖率没有显著差异。与 TGFβ1 组相比，TGFβ1+OE 处理的细胞表现出明显降低的波形蛋白表达和增加的 E-钙粘蛋白表达（P<0.05）。
OE 通过 TGFb1 诱导的 EMT REC 形态、生长动力学和蛋白表达标志物（E-钙粘蛋白和波形蛋白）抑制 EMT 的能力，OE 和 TGFβ1 处理后显示出 EMT 的抑制作用。因此，本研究可为 OE 抑制病理性组织重塑和持续炎症的治疗潜力提供新的见解。
