Fogli Stefano, Arena Chiara, Carpi Sara, Polini Beatrice, Bertini Simone, Digiacomo Maria, Gado Francesca, Saba Alessandro, Saccomanni Giuseppe, Breschi Maria Cristina, Nieri Paola, Manera Clementina, Macchia Marco
a Department of Pharmacy , University of Pisa , Pisa , Italy.
b Interdepartmental Research Center "Nutraceuticals and Food for Health" University of Pisa , Pisa , Italy.
Nutr Cancer. 2016 Jul;68(5):873-7. doi: 10.1080/01635581.2016.1180407. Epub 2016 Jun 7.
Oleocanthal is one of the phenolic compounds of extra virgin olive oil with important anti-inflammatory properties. Although its potential anticancer activity has been reported, only limited evidence has been provided in cutaneous malignant melanoma. The present study is aimed at investigating the selective in vitro antiproliferative activity of oleocanthal against human malignant melanoma cells. Since oleocanthal is not commercially available, it was obtained as a pure standard by direct extraction and purification from extra virgin olive oil. Cell viability experiments carried out by WST-1 assay demonstrated that oleocanthal had a remarkable and selective activity for human melanoma cells versus normal dermal fibroblasts with IC50s in the low micromolar range of concentrations. Such an effect was paralleled by a significant inhibition of ERK1/2 and AKT phosphorylation and downregulation of Bcl-2 expression. These findings may suggest that extra virgin olive oil phenolic extract enriched in oleocanthal deserves further investigation in skin cancer.
油橄榄苦素是特级初榨橄榄油中的酚类化合物之一，具有重要的抗炎特性。尽管已有报道称其具有潜在的抗癌活性，但在皮肤恶性黑色素瘤方面仅提供了有限的证据。本研究旨在调查油橄榄苦素对人恶性黑色素瘤细胞的选择性体外抗增殖活性。由于油橄榄苦素没有商业供应，通过直接从特级初榨橄榄油中提取和纯化获得了纯标准品。通过WST-1法进行的细胞活力实验表明，油橄榄苦素对人黑色素瘤细胞具有显著的选择性活性，而对正常真皮成纤维细胞的IC50处于低微摩尔浓度范围。这种作用伴随着ERK1/2和AKT磷酸化的显著抑制以及Bcl-2表达的下调。这些发现可能表明，富含油橄榄苦素的特级初榨橄榄油酚类提取物值得在皮肤癌方面进行进一步研究。
