Mazlina M, Khairani-Bejo S, Hazilawati H, Tiagarahan T, Shaqinah N N, Zamri-Saad M
Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
BMC Vet Res. 2018 Jun 25;14(1):203. doi: 10.1186/s12917-018-1533-x.
This study was conducted to investigate the pathological changes and distribution of B. melitensis in the urinary tract of pregnant goats following acute experimental infection. Six Jamnapari crossbred does in their third trimester of pregnancy were randomly assigned into two groups; Group 1 was uninfected control and Group 2 was inoculated conjunctival with 0.1 mL of the inoculums containing 10 cfu/mL of live B. melitensis. All does were sacrificed 30 days post-inoculation before the kidney, ureter, urinary bladder, urethra and vaginal swab were collected for isolation of B. melitensis. The same tissue samples were fixed in 10% neutral buffered formalin for hematoxylin and eosin, and immunoperoxidase staining.
None of the goats showed clinical signs or gross lesions. The most consistent histopathology finding was the infiltration of mononuclear cells, chiefly the macrophages with few lymphocytes and occasionally neutrophils in all organs along the urinary tract of the infected goats of Group 2. Other histopathology findings included mild necrosis of the epithelial cells of the renal tubules, congestion and occasional haemorrhages in the various tissues. Kidneys showed the most severe lesions. Immunoperoxidase staining revealed the presence of B. melitensis within the infiltrating macrophages and the epithelium of renal tubules, ureter, urethra and urinary bladder. Most extensive distribution was observed in the urinary bladder. Brucella melitensis was successfully isolated at low concentration (3.4 × 10 cfu/g) in the various organs of the urinary tract and at high concentration (2.4 × 10 cfu/mL) in the vaginal swabs of all infected goats. Although B. melitensis was successfully isolated from the various organs of the urinary tract, it was not isolated from the urine samples that were collected from the urinary bladder at necropsy.
This study demonstrates the presence of low concentrations of B. melitensis in the organs of urinary tract of pregnant does, resulting in mild histopathology lesions. However, B. melitensis was not isolated from the urine that was collected from the urinary bladder.
本研究旨在调查急性实验性感染后怀孕山羊泌尿道中布鲁氏菌的病理变化及分布情况。将6只处于妊娠晚期的贾纳帕里杂交母羊随机分为两组；第1组为未感染对照组，第2组经结膜接种0.1 mL含10 cfu/mL活布鲁氏菌的接种物。接种后30天处死所有母羊，采集肾脏、输尿管、膀胱、尿道和阴道拭子以分离布鲁氏菌。将相同的组织样本固定在10%中性缓冲福尔马林中，进行苏木精-伊红染色和免疫过氧化物酶染色。
所有山羊均未表现出临床症状或肉眼可见病变。最一致的组织病理学发现是，在第2组受感染山羊泌尿道的所有器官中，单核细胞浸润，主要是巨噬细胞，伴有少量淋巴细胞，偶尔还有中性粒细胞。其他组织病理学发现包括肾小管上皮细胞轻度坏死、各组织充血和偶尔出血。肾脏病变最为严重。免疫过氧化物酶染色显示，在浸润的巨噬细胞以及肾小管、输尿管、尿道和膀胱的上皮细胞中存在布鲁氏菌。在膀胱中观察到的分布最为广泛。在所有受感染山羊的泌尿道各器官中均成功分离出低浓度（3.4×10 cfu/g）的布鲁氏菌，在阴道拭子中分离出高浓度（2.4×10 cfu/mL）的布鲁氏菌。虽然从泌尿道各器官中成功分离出了布鲁氏菌，但在尸检时从膀胱采集的尿液样本中未分离出该菌。
本研究表明，怀孕母羊泌尿道器官中存在低浓度的布鲁氏菌，导致轻度组织病理学病变。然而，从膀胱采集的尿液中未分离出布鲁氏菌。
