体内成像研究流感病毒感染小鼠肺部的病理生理变化和中性粒细胞动力学。
In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs.
机构信息
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, 108-8639 Tokyo, Japan.
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711.
出版信息
Proc Natl Acad Sci U S A. 2018 Jul 10;115(28):E6622-E6629. doi: 10.1073/pnas.1806265115. Epub 2018 Jun 25.
The pathophysiological changes that occur in lungs infected with influenza viruses are poorly understood. Here we established an in vivo imaging system that combines two-photon excitation microscopy and fluorescent influenza viruses of different pathogenicity. This approach allowed us to monitor and correlate several parameters and physiological changes including the spread of infection, pulmonary permeability, pulmonary perfusion speed, number of recruited neutrophils in infected lungs, and neutrophil motion in the lungs of live mice. Several physiological changes were larger and occurred earlier in mice infected with a highly pathogenic H5N1 influenza virus compared with those infected with a mouse-adapted human strain. These findings demonstrate the potential of our in vivo imaging system to provide novel information about the pathophysiological consequences of virus infections.
流感病毒感染肺部后发生的病理生理变化知之甚少。在这里，我们建立了一个体内成像系统，该系统结合了双光子激发显微镜和具有不同致病性的荧光流感病毒。这种方法使我们能够监测和关联包括感染传播、肺通透性、肺灌注速度、感染肺部募集的中性粒细胞数量以及活鼠肺部中性粒细胞运动在内的多个参数和生理变化。与感染适应小鼠的人源株相比，感染高致病性 H5N1 流感病毒的小鼠发生的几种生理变化更大且更早。这些发现表明，我们的体内成像系统具有提供有关病毒感染病理生理后果的新信息的潜力。
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