Henry I, Gallano P, Besmond C, Weil D, Mattei M G, Turleau C, Boué J, Kahn A, Junien C
Ann Hum Genet. 1985 Jul;49(3):173-80. doi: 10.1111/j.1469-1809.1985.tb01691.x.
We used a cloned cDNA probe for the B subunit of human aldolase (ALDB) and Southern blotting techniques to analyse DNA from a series of rodent X human somatic cell hybrids for the presence of specific ALDB-related sequences. Our results provide evidence for the assignment of the gene for ALDB to chromosome 9. Moreover, by direct gene dosage determination in two patients with chromosome 9 unbalanced rearrangements and by in situ hybridization we refined the regional chromosomal assignment to 9q13----q32 and most probably to 9q21.3----9q22.2.
我们使用人醛缩酶(ALDB)B亚基的克隆cDNA探针和Southern印迹技术,分析了一系列啮齿动物X人类体细胞杂种的DNA,以检测是否存在特定的与ALDB相关的序列。我们的结果为将ALDB基因定位于9号染色体提供了证据。此外,通过对两名9号染色体不平衡重排患者进行直接基因剂量测定以及原位杂交,我们将该基因在染色体上的定位区域精确到了9q13----q32,很可能是9q21.3----9q22.2。