Key Laboratory of Chem-Biosensing, Anhui Province; Key Laboratory of Functional Molecular Solids, Anhui Province; College of Chemistry and Materials Science , Anhui Normal University , Wuhu 241000 , P. R. China.
Institute of Molecular Biology and Biotechnology and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences , Anhui Normal University , Wuhu 241000 , P. R. China.
ACS Sens. 2018 Jul 27;3(7):1368-1375. doi: 10.1021/acssensors.8b00304. Epub 2018 Jul 16.
Despite some recent developments on the portable on-site sensor of Aflatoxin B1 (AFB1), the complex and expensive preparation of recognition elements have still limited their wide applications. In this paper, using the fast, low-cost, and stable recognition of aptamer DNA-AFB1, a portable aptasensor was constructed for the on-site detection of AFB1 in food matrixes, with the readout of personal glucose meter (PGM) and DNA walking machine for signal probe separation. In such an assay protocol, the target could trigger the DNA walker to autonomously move on the electrode surface, propelled by unidirectional Pb-specific DNAzyme digestion, which could amplify the signal and separate the signal probe as well for further quantification by the PGM. Under optimized conditions, the increase of PGM signal was relative with the concentration of AFB1 ranging from 0.02 to 10 nM and the low limit of detection (LOD) was 10 pM (S/N = 3). With the features of portability, and cheapness, the presented user-friendly method could be extended to various other analytes for wide point-of-care applications.
尽管便携式现场传感器在黄曲霉毒素 B1（AFB1）方面取得了一些新进展，但识别元件的复杂和昂贵的制备仍然限制了它们的广泛应用。在本文中，我们利用适体 DNA-AFB1 的快速、低成本和稳定识别，构建了一种用于现场检测食品基质中 AFB1 的便携式适体传感器，其读出设备为个人血糖仪（PGM）和 DNA 行走机，用于信号探针分离。在这种分析方案中，目标可以触发 DNA 行走器在电极表面上自主移动，由单向 Pb 特异性 DNA 酶的消化来推动，从而可以放大信号并分离信号探针，然后通过 PGM 进行进一步定量。在优化条件下，PGM 信号的增加与 AFB1 的浓度在 0.02 至 10 nM 范围内呈正相关，检测限（LOD）低至 10 pM（S/N = 3）。由于具有便携性和经济性的特点，本研究提出的用户友好型方法可以扩展到其他各种分析物，以实现广泛的即时检测应用。
