Li Tian, Pan Rui, Wen Yuhan, Xu Jiaqi, Zhang Liping, He Suna, Liang Gaofeng
School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang 471023, China.
Biosensors (Basel). 2022 Apr 15;12(4):249. doi: 10.3390/bios12040249.
A simple, selective, and quantitative platform for point-of-care diagnostic of COVID-19 is urgently needed as a complement in areas where resources are currently relatively scarce. To meet the needs of early diagnosis and intervention, a proof-of-concept demonstration of a universal personal glucose meter-based nucleic acid assay platform (PGM-NAAP) is presented, which converts to SARS-CoV-2 detection from glucose detection. By using magnetic bead separation together with the hand-held PGM for quantitative readout, PGM-NAAP achieves the 98 pM limit of detection for a sequence related to SARS-CoV-2. The ability to discriminate target nucleic acid from genomic DNA, the satisfactory spike recoveries of saliva and serum samples, as well as the good stability all together suggest the potential of the PGM-NAAP for the screening and diagnosis of suspected patients during the outbreaks of COVID-19 in resource-limited settings without sophisticated instruments. On the basis of these findings, PGM-NAAP can be expected to provide an accurate and convenient path for diagnosis of disease-associated nucleic acid.
在当前资源相对匮乏的地区，迫切需要一个简单、选择性强且定量的即时护理诊断平台，作为一种补充手段。为满足早期诊断和干预的需求，本文展示了一种基于通用血糖仪的核酸检测平台（PGM - NAAP）的概念验证示范，该平台可从葡萄糖检测转换为SARS-CoV-2检测。通过使用磁珠分离技术以及手持式PGM进行定量读数，PGM - NAAP对与SARS-CoV-2相关的序列实现了98 pM的检测限。从基因组DNA中区分目标核酸的能力、唾液和血清样本令人满意的加标回收率以及良好的稳定性，共同表明了PGM - NAAP在资源有限且无精密仪器的环境中，对COVID-19疫情期间疑似患者进行筛查和诊断的潜力。基于这些发现，有望PGM - NAAP为疾病相关核酸的诊断提供一条准确且便捷的途径。
