Zhang Bowen, Li Changhui, Zhang Hang, Chen Yun, Jiang Hui, Chen Leifeng, Ur Rehman Fawad, Wang Xuemei
J Biomed Nanotechnol. 2018 Jul 1;14(7):1277-1286. doi: 10.1166/jbn.2018.2586.
A novel dopamine biosensor based on carbon fiber microelectrodes (CFMEs) modified with copper(I) sulfide functionalized nanocomposites of the reduced graphene oxide (Cu2S/RGO) has been explored for the sensitive detection of dopamine and in vivo monitoring the neurotransmitters released by Drosophila's brain. The as-prepared Cu2S/RGO decorated microelectrodes were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry (CV). Our observations demonstrate that Cu2S/RGO-CFMEs exhibited excellent catalytic activity and high selectivity for dopamine with relatively low detection limit (24 nM), wide linear range (i.e., from 0.1-20 μM) and outstanding reproducibility. Furthermore, the as-prepared new dopamine biosensor with high sensitivity and good stability was readily used to detect the amount of dopamine in the brain of drosophila, indicating the potential and promising application in the in vivo measurement of neurotransmitters without other electrochemical interference such as histidine, ascorbic acid, uric acid, and others.
一种基于用硫化亚铜功能化的还原氧化石墨烯（Cu2S/RGO）修饰的碳纤维微电极（CFMEs）的新型多巴胺生物传感器，已被用于灵敏检测多巴胺以及在体内监测果蝇大脑释放的神经递质。通过扫描电子显微镜（SEM）、X射线光电子能谱（XPS）和循环伏安法（CV）对所制备的Cu2S/RGO修饰微电极进行了表征。我们的观察结果表明，Cu2S/RGO-CFMEs对多巴胺表现出优异的催化活性和高选择性，检测限相对较低（24 nM），线性范围宽（即从0.1 - 20 μM）且重现性出色。此外，所制备的具有高灵敏度和良好稳定性的新型多巴胺生物传感器易于用于检测果蝇大脑中的多巴胺含量，表明其在无其他电化学干扰（如组氨酸、抗坏血酸、尿酸等）的神经递质体内测量中具有潜在且有前景的应用。
