Department of Micro- and Nanotechnology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Department of Chemistry, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Biosensors (Basel). 2018 Jul 19;8(3):70. doi: 10.3390/bios8030070.
An enzyme-based electrochemical biosensor has been developed with 3D pyrolytic carbon microelectrodes that have been coated with bio-functionalized reduced graphene oxide (RGO). The 3D carbon working electrode was microfabricated using the pyrolysis of photoresist precursor structures, which were subsequently functionalized with graphene oxide and enzymes. Glucose detection was used to compare the sensor performance achieved with the 3D carbon microelectrodes (3DCMEs) to the 2D electrode configuration. The 3DCMEs provided an approximately two-fold higher sensitivity of 23.56 µA·mM·cm compared to 10.19 µA mM·cm for 2D carbon in glucose detection using cyclic voltammetry (CV). In amperometric measurements, the sensitivity was more than 4 times higher with 0.39 µA·mM·cm for 3D electrodes and 0.09 µA·mM·cm for the 2D configuration. The stability analysis of the enzymes on the 3D carbon showed reproducible results over 7 days. The selectivity of the electrode was evaluated with solutions of glucose, uric acid, cholesterol and ascorbic acid, which showed a significantly higher response for glucose.
一种基于酶的电化学生物传感器已经开发出来，该传感器使用涂有生物功能化还原氧化石墨烯（RGO）的 3D 热解碳微电极。3D 碳工作电极是通过光致抗蚀剂前体结构的热解微制造的，随后用氧化石墨烯和酶对其进行功能化。使用循环伏安法（CV）进行葡萄糖检测，比较了 3D 碳微电极（3DCME）和 2D 电极构型的传感器性能。与 2D 碳相比，3DCME 在葡萄糖检测中提供了约两倍高的灵敏度，为 23.56 µA·mM·cm，而 2D 碳的灵敏度为 10.19 µA mM·cm。在安培测量中，3D 电极的灵敏度超过 4 倍，为 0.39 µA·mM·cm，而 2D 构型的灵敏度为 0.09 µA·mM·cm。对 3D 碳上酶的稳定性分析表明，在 7 天内可重复获得结果。通过葡萄糖、尿酸、胆固醇和抗坏血酸溶液评估了电极的选择性，结果表明葡萄糖的响应明显更高。
