正常血压大鼠和自发性高血压大鼠星状神经节的转录谱分析。
Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains.
机构信息
Burdon Sanderson Cardiac centre, Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, OX1 3PT, UK.
OXION initiative, Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, OX1 3PT, UK.
出版信息
Sci Data. 2018 Jun 26;5:180123. doi: 10.1038/sdata.2018.123.
The course of hypertension remains poorly understood, although impairment of the sympathetic nervous systems is thought to play a role in its aetiology. In this study, RNA-sequencing (RNAseq) was used to identify transcriptomal differences in the sympathetic stellate ganglia between 16-week-old normotensive Wistar rats and spontaneously hypertensive rats (SHR). Sequencing quality was assessed by FastQC and quasi-mapping rate by Salmon. Differential expression results were confirmed by real time reverse transcriptase Quantitative Polymerase Chain Reaction (qRT-PCR). RNAseq analysis was found to be predictive and representative of transcriptomal changes when compared to qRT-PCR by correlation analysis. Whether these changes underpin physiological sympathetic phenotypes associated with hypertension remains to be established, however this dataset identifies lead transcripts as a priori targets for further investigation.
高血压的发病机制仍不清楚，尽管人们认为交感神经系统的损伤在其发病机制中起作用。在这项研究中，我们使用 RNA 测序（RNAseq）来鉴定 16 周龄正常血压 Wistar 大鼠和自发性高血压大鼠（SHR）之间交感星状神经节中的转录组差异。通过 FastQC 评估测序质量，通过 Salmon 评估准映射率。通过实时逆转录定量聚合酶链反应（qRT-PCR）确认差异表达结果。通过相关性分析发现，与 qRT-PCR 相比，RNAseq 分析具有预测性和转录组变化的代表性。然而，这些变化是否支持与高血压相关的生理性交感表型尚待确定，但该数据集确定了主要转录物作为进一步研究的先验靶标。
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