Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, 1111, Budapest, Hungary.
Fermentia Microbiological Ltd., Berlini út 47-49, 1045, Budapest, Hungary.
Chembiochem. 2018 Sep 4;19(17):1845-1848. doi: 10.1002/cbic.201800286. Epub 2018 Jul 30.
An improved sol-gel process involving the use of hollow silica microspheres as a supporting additive was applied for the co-immobilization of whole cells of Escherichia coli with Chromobacterium violaceum ω-transaminase activity and Lodderomyces elongisporus with ketoreductase activity. The co-immobilized cells with two different biocatalytic activities could perform a cascade of reactions to convert racemic 4-phenylbutan-2-amine or heptan-2-amine into a nearly equimolar mixture of the corresponding enantiomerically pure R amine and S alcohol even in continuous-flow mode. The novel co-immobilized whole-cell system proved to be an easy-to-store and durable biocatalyst.
采用一种改进的溶胶-凝胶法，使用中空二氧化硅微球作为支撑添加剂，共同固定具有色杆菌ω-转氨酶活性的大肠杆菌全细胞和长形埃默森酵母具有酮还原酶活性的全细胞。具有两种不同生物催化活性的共固定化细胞可以进行级联反应，即使在连续流动模式下，也可以将外消旋 4-苯基丁-2-胺或庚-2-胺转化为相应对映体纯 R 胺和 S 醇的近乎等摩尔混合物。新型共固定化全细胞体系被证明是一种易于储存和耐用的生物催化剂。
