Lowance Center for Human Immunology, Division of Rheumatology, Department of Medicine, Emory University School of Medicine, Atlanta, GA.
Informatics Institute, University of Alabama at Birmingham, Birmingham, AL.
Immunol Rev. 2018 Jul;284(1):120-131. doi: 10.1111/imr.12660.
Understanding antibody repertoires and in particular, the properties and fates of B cells expressing potentially pathogenic antibodies is critical to define the mechanisms underlying multiple immunological diseases including autoimmune and allergic conditions as well as transplant rejection. Moreover, an integrated knowledge of the antibody repertoires expressed by B cells and plasma cells (PC) of different functional properties and longevity is essential to develop new therapeutic strategies, better biomarkers for disease segmentation, and new assays to measure restoration of B-cell tolerance or, at least, of normal B-cell homeostasis. Reaching these goals, however, will require a more precise phenotypic, functional and molecular definition of B-cell and PC populations, and a comprehensive analysis of the antigenic reactivity of the antibodies they express. While traditionally hampered by technical and ethical limitations in human experimentation, new technological advances currently enable investigators to address these questions in a comprehensive fashion. In this review, we shall discuss these concepts as they apply to the study of Systemic Lupus Erythematosus.
理解抗体库，特别是表达潜在致病性抗体的 B 细胞的特性和命运，对于定义多种免疫性疾病（包括自身免疫和过敏疾病以及移植排斥）的机制至关重要。此外，对不同功能特性和寿命的 B 细胞和浆细胞（PC）表达的抗体库进行综合了解，对于开发新的治疗策略、更好的疾病细分生物标志物以及新的检测方法来衡量 B 细胞耐受的恢复或至少恢复正常 B 细胞的体内平衡至关重要。然而，要实现这些目标，需要更精确地表征、功能和分子定义 B 细胞和 PC 群体，并全面分析它们所表达的抗体的抗原反应性。尽管传统上受到人类实验中的技术和伦理限制，但新的技术进步目前使研究人员能够全面地解决这些问题。在这篇综述中，我们将讨论这些概念在系统性红斑狼疮研究中的应用。
