School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China.
Chongqing Tuberculosis Control Institute, Chongqing 400050, China.
Microb Pathog. 2018 Oct;123:36-41. doi: 10.1016/j.micpath.2018.06.036. Epub 2018 Jun 23.
Rcs phosphorelay system is a two-component signal transduction system, which can regulate the transcription of capsule polysaccharide and biofilm related genes in Enterobacteriaceae. In this study, microarray technology was used to investigate the overall genes regulated by RcsA, RcsB, and RcsAB and the regulation mechanism in Klebsiella pneumoniae, then COG analysis was performed to explore the functions of the differentially expressed genes. According to the microarray data result, a total of 45, 223 and 217 genes regulated by RcsA, RcsB, and RcsAB were screened. The result of COG analysis suggested that inorganic ion transport and metabolism related genes have a majority in RcsA regulating genes. Most of RcsB regulated genes were showed involved in energy production and conversion process. Besides Carbohydrate transport and metabolism genes were identified as the major components of the RcsAB regulated genes. 15 differentially expressed genes were confirmed by quantitative real-time PCR (RT-qPCR). The RT-qPCR results indicated that 13 genes consistent with microarray data. The results of this study provided important evidence for further research to investigate the influence of RcsA, RcsB, RcsAB regulators and further efforts to address the diseased caused by K.pneumoniae, such as pneumonia, bacteremia, and urinary tract infection.
Rcs 磷酸传递系统是一种双组分信号转导系统，可调节肠杆菌科中荚膜多糖和生物膜相关基因的转录。在本研究中，使用微阵列技术研究了 RcsA、RcsB 和 RcsAB 调节的整体基因以及肺炎克雷伯菌中的调节机制，然后进行了 COG 分析以探索差异表达基因的功能。根据微阵列数据结果，筛选出 RcsA、RcsB 和 RcsAB 分别调节的 45、223 和 217 个基因。COG 分析的结果表明，无机离子转运和代谢相关基因在 RcsA 调节基因中占多数。RcsB 调节的大多数基因都参与了能量产生和转化过程。此外，碳水化合物转运和代谢基因被确定为 RcsAB 调节基因的主要组成部分。通过定量实时 PCR (RT-qPCR) 验证了 15 个差异表达基因。RT-qPCR 结果表明，有 13 个基因与微阵列数据一致。本研究的结果为进一步研究 RcsA、RcsB、RcsAB 调节剂的影响提供了重要证据，并进一步努力解决肺炎克雷伯菌引起的疾病，如肺炎、菌血症和尿路感染。
