College of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscaping, Ministry of Agriculture, Nanjing 210095, China.
College of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscaping, Ministry of Agriculture, Nanjing 210095, China.
Gene. 2018 Oct 20;674:161-169. doi: 10.1016/j.gene.2018.06.075. Epub 2018 Jun 23.
RNA-Seq was applied to capture the transcriptome of the leaf and root of non-treated and salinity-treated chrysanthemum cv. 'Jinba' plants. A total of 206,868 unigenes of mean length 849 nt and of N50 length 1363 nt was identified; of these about 64% (> 132,000) could be functionally assigned. Depending on the severity of the salinity stress, differential transcription was observed for genes encoding proteins involved in osmotic adjustment, in ion transport, in reactive oxygen species scavenging and in the regulation of abscisic acid (ABA) signaling. The root stress response was dominated by the up-regulation of genes involved in ion transport, while that of the leaf reflected the plant's effort to make osmotic adjustments and to regulate Ca transport. An array of known transcription factors (WRKY, AP2/ERF, MYB, bHLH and NAC) were differentially transcribed.
RNA-Seq 被应用于捕获未经处理和盐处理的菊花 cv. 'Jinba' 植物叶片和根部的转录组。共鉴定出 206,868 条平均长度为 849nt、N50 长度为 1363nt 的 unigenes；其中约 64%（>132,000 条）可被赋予功能。根据盐胁迫的严重程度，参与渗透调节、离子转运、活性氧清除和脱落酸（ABA）信号调节的蛋白质编码基因的转录出现差异。根的应激反应主要由参与离子转运的基因上调所主导，而叶的应激反应则反映了植物进行渗透调节和钙运输调节的努力。一系列已知的转录因子（WRKY、AP2/ERF、MYB、bHLH 和 NAC）的转录出现差异。
