College of Landscape Architecture, Northeast Forestry University, No. 26 Hexing Road, Harbin, 150006, Heilongjiang, China.
College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, Jilin, China.
BMC Plant Biol. 2022 May 12;22(1):240. doi: 10.1186/s12870-022-03612-x.
As high soil salinity threatens the growth and development of plants, understanding the mechanism of plants' salt tolerance is critical. The Chrysanthemum × grandiflora is a newly developed species with a strong salt resistance that possesses multiple genes controlling its quantitative salt resistance. Because of this multigene control, we chose to investigate the plant stress genes overall responses at the transcriptome level. C. grandiflora were treated with a 200 mM NaCl solution for 12 h to study its effect on the roots and leaves via Illumina RNA sequencing. PAL, CYP73A, and 4CL in the phenylpropanoid biosynthesis pathway were upregulated in roots and leaves. In the salicylic acid signal transduction pathway, TGA7 was upregulated in the roots and leaves, while in the jasmonic acid signal transduction pathway, TIFY9 was upregulated in the roots and leaves. In the ion transporter gene, we identified HKT1 that showed identical expression patterns in the roots and leaves. The impact of NaCl imposition for 12 h was largely due to osmotic effect of salinity on C. grandiflora, and most likely the transcript abundance changes in this study were due to the osmotic effect. In order to verify the accuracy of the Illumina sequencing data, we selected 16 DEGs for transcription polymerase chain reaction (qRT-PCR) analysis. qRT-PCR and transcriptome sequencing analysis revealed that the transcriptome sequencing results were reliable.
由于高土壤盐度会威胁植物的生长和发育，因此了解植物耐盐性的机制至关重要。菊花是一种新开发的具有强耐盐性的物种，具有控制其定量耐盐性的多个基因。由于这种多基因控制，我们选择在转录组水平上研究植物应激基因的整体反应。用 200 mM NaCl 溶液处理菊花 12 小时，通过 Illumina RNA 测序研究其对根和叶的影响。苯丙烷生物合成途径中的 PAL、CYP73A 和 4CL 在根和叶中上调。在水杨酸信号转导途径中，TGA7 在根和叶中上调，而在茉莉酸信号转导途径中，TIFY9 在根和叶中上调。在离子转运基因中，我们鉴定出 HKT1，它在根和叶中表现出相同的表达模式。NaCl 处理 12 小时的影响主要是由于盐度对菊花的渗透作用，而且本研究中转录丰度的变化很可能是由于渗透作用所致。为了验证 Illumina 测序数据的准确性，我们选择了 16 个差异表达基因进行转录聚合酶链反应（qRT-PCR）分析。qRT-PCR 和转录组测序分析表明，转录组测序结果可靠。
