Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.
BMC Plant Biol. 2020 Mar 14;20(1):114. doi: 10.1186/s12870-020-02330-6.
Areas with saline soils are sparsely populated and have fragile ecosystems, which severely restricts the sustainable development of local economies. Zoysia grasses are recognized as excellent warm-season turfgrasses worldwide, with high salt tolerance and superior growth in saline-alkali soils. However, the mechanism underlying the salt tolerance of Zoysia species remains unknown.
The phenotypic and physiological responses of two contrasting materials, Zoysia japonica Steud. Z004 (salt sensitive) and Z011 (salt tolerant) in response to salt stress were studied. The results show that Z011 was more salt tolerant than was Z004, with the former presenting greater K/Na ratios in both its leaves and roots. To study the molecular mechanisms underlying salt tolerance further, we compared the transcriptomes of the two materials at different time points (0 h, 1 h, 24 h, and 72 h) and from different tissues (leaves and roots) under salt treatment. The 24-h time point and the roots might make significant contributions to the salt tolerance. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in the salt-stress response belonged to the hormone pathway, various TF families and the DUF family.
Zoysia salt treatment transcriptome shows the 24-h and roots may make significant contributions to the salt tolerance. The auxin signal transduction family, ABA signal transduction family, WRKY TF family and bHLH TF family may be the most important families in Zoysia salt-stress regulation.
盐渍土地区人口稀少，生态系统脆弱，严重制约着当地经济的可持续发展。结缕草是世界范围内公认的优良暖季型草坪草，具有较高的耐盐性和在盐碱土中的良好生长能力。然而，结缕草属物种的耐盐机制尚不清楚。
研究了两个具有鲜明对比的材料，结缕草（盐敏感）Z004 和 Z011（盐耐受）对盐胁迫的表型和生理响应。结果表明，Z011 比 Z004 更耐盐，前者在叶片和根部的 K/Na 比值都更大。为了进一步研究耐盐的分子机制，我们比较了两种材料在不同时间点（0 h、1 h、24 h 和 72 h）和不同组织（叶片和根部）在盐处理下的转录组。24 h 时间点和根部可能对耐盐性有重要贡献。此外，不同比较的 GO 和 KEGG 分析表明，参与盐胁迫响应的关键差异表达基因属于激素途径、各种 TF 家族和 DUF 家族。
结缕草盐处理转录组显示，24 h 和根部可能对耐盐性有重要贡献。生长素信号转导家族、ABA 信号转导家族、WRKY TF 家族和 bHLH TF 家族可能是结缕草盐胁迫调节中最重要的家族。
