Terrestrial Ecology, Institute of Zoology, University of Cologne, Zülpicher Str. 47b, 50674, Köln, Germany.
Department of Zoology, Institute of Biosciences, University of São Paulo, Rua do Matão, tv. 14, 101, 05508-090, São Paulo, Brazil.
J Eukaryot Microbiol. 2019 Mar;66(2):232-243. doi: 10.1111/jeu.12665. Epub 2018 Jul 8.
A major drawback in testate amoeba research is a general lack of scientific studies combining molecular approaches and classical laboratory experiments. We isolated five yet uncultured testate amoebae of the genus Phryganella Penard, 1902 from three different rivers and one pond in Germany. Based on established cultures we show their morphology, which we studied by light and electron microscopy, and present their unique feeding mode on abundant and common pennate diatoms like Nitzschia spp. and Synedra spp., whose frustules are bent and frequently, but not always, broken during the feeding process. We further obtained the first SSU rDNA sequences of strains of the family Phryganellidae, all of which contain introns. We used the sequences to confirm the taxonomic placement of the Phryganellidae in the Arcellinida (Amoebozoa), branching as a sister group to the Cryptodifflugiidae.
在研究有壳变形虫时，主要存在一个缺陷，即缺乏将分子方法与经典实验室实验相结合的科学研究。我们从德国的三条不同的河流和一个池塘中分离出了五个尚未培养的有壳变形虫属 Phryganella Penard，1902。基于已建立的培养方法，我们展示了它们的形态，通过光学显微镜和电子显微镜进行了研究，并介绍了它们在丰富且常见的羽纹硅藻属（Nitzschia spp. 和 Synedra spp.）上的独特摄食方式，这些硅藻的壳片在摄食过程中被弯曲且经常但并非总是被折断。我们进一步获得了 Phryganellidae 家族菌株的首个 SSU rDNA 序列，所有这些序列都包含内含子。我们使用这些序列来确认 Phryganellidae 在 Arcellinida（变形虫）中的分类地位，作为 Cryptodifflugiidae 的姐妹群分支。
