University of Cologne, Terrestrial Ecology, Institute of Zoology, Zülpicher Str. 47b, 50674 Köln, Germany.
University of Cologne, Terrestrial Ecology, Institute of Zoology, Zülpicher Str. 47b, 50674 Köln, Germany.
Eur J Protistol. 2020 Aug;75:125707. doi: 10.1016/j.ejop.2020.125707. Epub 2020 May 19.
Phryganella acropodia Hertwig and Lesser, 1874, is one of the most common and abundant testate amoeba species. It represents the type species of the genus Phryganella Penard, 1902, which in turn is the type genus for the suborder Phryganellina (Arcellinida) Bovee, 1985, but despite its taxonomic importance it was not yet analyzed with molecular methods. We established two cultures of putative Phryganella acropodia, designed Phryganellina-specific primers, amplified SSU rDNA data and subjected these sequences to phylogenetic analyses. Morphological and genetic differences were found between both strains. With SSU rDNA phylogenetic analyses we confirm that Phryganella acropodia branches with Phryganella paradoxa Penard, 1902 and Cryptodifflugia Penard, 1890 in the Phryganellina. We thus give further evidence that pseudopodia morphology in the Arcellinida is a character of high taxonomic value, as suggested by Bovee and Jung when erecting the suborder Phryganellina. Moreover, we provide evidence for cryptic diversity and for the first time confirm the existence of a naked life stage in Arcellinida by molecular means.
赫特维希和利塞 1874 年所描述的 Phryganella acropodia 是最常见和丰富的有壳变形虫物种之一。它代表 Phryganella 属的模式种，而 Phryganella 属又是亚目 Phryganellina（Arcellinida）的模式属，尽管具有分类学重要性，但尚未用分子方法进行分析。我们建立了两个假定的 Phryganella acropodia 培养物，设计了 Phryganellina 特异性引物，扩增了 SSU rDNA 数据，并对这些序列进行了系统发育分析。两个菌株之间存在形态和遗传差异。通过 SSU rDNA 系统发育分析，我们确认 Phryganella acropodia 与 Phryganella paradoxa Penard，1902 和 Cryptodifflugia Penard，1890 一起分支在 Phryganellina 中。因此，我们进一步证明了伪足形态在 Arcellinida 中具有高度分类价值，正如 Bovee 和 Jung 在建立 Phryganellina 亚目时所提出的那样。此外，我们提供了隐种多样性的证据，并首次通过分子手段证实了 Arcellinida 中存在裸露生活阶段。
