Kumar Satyanshu, Singh Raghuraj, Gajbhiye Narendra, Dhanani Tushar
Indian Council of Agricultural Research (ICAR), Directorate of Medicinal and Aromatic Plants Research, Boriavi, Anand, Gujarat, India 387310.
J AOAC Int. 2018 Nov 1;101(6):1773-1780. doi: 10.5740/jaoacint.18-0081. Epub 2018 Jun 26.
: Both the roots and leaves of are products of commerce. They contain active compounds of therapeutic value and mostly different withanolides. Several pharmacological activities of have links to one or more withanolides. The presence of phenolic compounds in extracts could play a vital role in the reduction of blood glucose levels in diabetic subjects. : The present study was carried out for the selection of a solvent to prepare extracts rich in phenolics, withaferin A (WA), 12-deoxywithastromonolide (12WD), and withanolide A (WDA). A simple, rapid HPLC method was also developed for the identification and quantification of WA, 12WD, and WDA. : The extraction efficiency of aqueous alcoholic solvents including hexane, chloroform, ethyl acetate, and methanol were compared for three selected withanolides and total phenolic content. The contents of WA, 12WD, and WDA and total phenolics were determined in the extracts. The quality of nine formulations containing were also compared in terms of the content of WA, 12WD, and WDA and total phenolics. : The maximum extract yield and the total withanolide and phenolic content were obtained from aqueous alcoholic compositions at 50:50 (v/v), 70:30 (v/v), and 100:0 (v/v), respectively. In the case of organic solvents, chloroform and ethyl acetate yielded the highest concentrations of phenolics and three withanolides, respectively. The total phenolic content in formulations was in the range of 1.84-3.13%, and total withanolide content showed wide variability. : The outcome of the present investigation could be utilized for the selection of extraction solvents to prepare -enriched extracts and their quality monitoring by using the developed and validated HPLC-Photodiode array detection method. : A process for preparation of phenolics and withanolides (withaferin A, 12-deoxywithastramonolide and withanolide A) enriched extracts of . Simple and rapid HPLC method was also developed and validated as per the ICH guidelines for identification and quantification of three major withanolides. The developed HPLC method was applied to analyze the quality of extracts and marketed herbal products (mono, as well as poly constituents). Optimized extraction process could be utilized for upscaling process development in preparation of enriched extracts from , crop improvement, bio-prospection studies and quality control.
[植物名称]的根和叶都是商业产品。它们含有具有治疗价值的活性化合物，主要是不同的睡茄内酯。[植物名称]的几种药理活性与一种或多种睡茄内酯有关。提取物中酚类化合物的存在可能在降低糖尿病患者血糖水平方面发挥重要作用。
本研究旨在选择一种溶剂，以制备富含酚类、阿魏酸A（WA）、12-脱氧欧茄内酯（12WD）和睡茄内酯A（WDA）的提取物。还开发了一种简单、快速的高效液相色谱法，用于鉴定和定量WA、12WD和WDA。
比较了己烷、氯仿、乙酸乙酯和甲醇等水性醇类溶剂对三种选定睡茄内酯和总酚含量的提取效率。测定了提取物中WA、12WD和WDA的含量以及总酚含量。还比较了九种含[植物名称]制剂中WA、12WD和WDA的含量以及总酚含量。
在体积比为50:50（v/v）、70:30（v/v）和100:0（v/v）的水性醇类组合物中分别获得了最高提取物产率以及总睡茄内酯和酚类含量。就有机溶剂而言，氯仿和乙酸乙酯分别产生了最高浓度的酚类和三种睡茄内酯。制剂中的总酚含量在1.84 - 3.13%范围内，总睡茄内酯含量差异很大。
本研究结果可用于选择提取溶剂，以制备富含[植物名称]的提取物，并通过使用已开发和验证的高效液相色谱 - 光电二极管阵列检测方法对其质量进行监测。
一种制备富含酚类和睡茄内酯（阿魏酸A、12-脱氧欧茄内酯和睡茄内酯A）的[植物名称]提取物的方法。还根据国际协调会议（ICH）指南开发并验证了一种简单、快速的高效液相色谱法，用于鉴定和定量三种主要睡茄内酯。所开发的高效液相色谱法用于分析提取物和市售草药产品（单一成分以及多成分产品）的质量。优化的提取工艺可用于扩大从[植物名称]制备富集提取物的工艺开发规模、作物改良、生物勘探研究和质量控制。
