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采用高效液相色谱-漂移管-离子迁移四极杆飞行时间质谱法测定印度人参制品中的睡茄内酯和睡茄苷

Determination of Withanolides and Withanosides in Ashwagandha Based Products Using HPLC-Drift-Tube-Ion-Mobility Quadrupole Time-of-Flight Mass Spectrometry.

作者信息

Zellner Laura, Wierer Markus, Himmelsbach Markus, Schatzmann Benjamin, Thalhamer Bernhard, Klampfl Christian W

机构信息

Institute of Analytical and General Chemistry, Johannes Kepler University Linz, Linz, Austria.

出版信息

Electrophoresis. 2025 Mar;46(5-6):340-346. doi: 10.1002/elps.202400188. Epub 2025 Feb 18.

DOI:10.1002/elps.202400188
PMID:39965076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11952284/
Abstract

A total of 19 products based on extracts from Withania somnifera (L.) Dunal, better known by its more common name ashwagandha, and five products based on ashwagandha root powder were analyzed with respect to their content in the biologically relevant substances belonging to the group of withanolides and withanosides. Using HPLC coupled to drift-tube ion-mobility quadrupole time-of-flight mass spectrometry (DT-IM-QTOF-MS), 19 withanolides and withanosides could be tentatively identified. The comparison of the results from the quantitative analysis with the information on the product labels showed that the percentage of withanolides and withanosides deviated from the stated specifications by at least a factor of two and at most a factor of 35.

摘要

对总共19种基于睡茄（Withania somnifera (L.) Dunal）提取物（其更为人熟知的通用名称是南非醉茄）的产品以及5种基于南非醉茄根粉的产品，就其属于睡茄内酯类和睡茄糖苷类的生物活性相关物质含量进行了分析。使用与漂移管离子淌度四极杆飞行时间质谱联用的高效液相色谱法（DT-IM-QTOF-MS），初步鉴定出了19种睡茄内酯类和睡茄糖苷类物质。定量分析结果与产品标签信息的比较表明，睡茄内酯类和睡茄糖苷类的百分比与规定规格的偏差至少为2倍，最多为35倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/69ca74384d39/ELPS-46--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/12cb4b876b74/ELPS-46--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/9f7082604b8c/ELPS-46--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/69ca74384d39/ELPS-46--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/12cb4b876b74/ELPS-46--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/9f7082604b8c/ELPS-46--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b9/11952284/69ca74384d39/ELPS-46--g002.jpg

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Nucleic Acids Res. 2024 Jul 5;52(W1):W398-W406. doi: 10.1093/nar/gkae253.
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Pharmacokinetics and bioequivalence of (Ashwagandha) extracts - A double blind, crossover study in healthy adults.（印度人参）提取物的药代动力学和生物等效性——一项在健康成年人中的双盲交叉研究。
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