Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Carl Neuberg-Str. 1, 30625 Hannover, Germany; Cluster of Excellence "Hearing4all" of the German Research Foundation, Germany.
Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Carl Neuberg-Str. 1, 30625 Hannover, Germany.
Hear Res. 2018 Oct;368:109-122. doi: 10.1016/j.heares.2018.06.015. Epub 2018 Jun 21.
Transplantation of mesenchymal stromal cells (MSC) presents a promising approach not only for the replacement of lost or degenerated cells in diseased organs but also for local drug delivery. It can potentially be used to enhance the safety and efficacy of inner ear surgeries such as cochlear implantation. Options for enhancing the effects of MSC therapy include modulating cell behaviour with customized bio-matrixes or modulating their behaviour by ex vivo transfection of the cells with a variety of genes. In this study, we demonstrate that MSC delivered to the inner ear of guinea pigs or to decellularized cochleae preferentially bind to areas of high heparin concentration. This presents an opportunity for modulating cell behaviour ex vivo. We evaluated the effect of carboxymethylglucose sulfate (Cacicol), a heparan sulfate analogue on spiral ganglion cells and MSC and demonstrated support of neuronal survival and support of stem cell proliferation.
间质基质细胞（MSC）的移植不仅为患病器官中丢失或退化的细胞的替代提供了一种很有前途的方法，而且还为局部药物输送提供了一种很有前途的方法。它可能被用于增强内耳手术（如人工耳蜗植入术）的安全性和有效性。增强 MSC 治疗效果的选择包括通过定制生物基质来调节细胞行为，或通过用各种基因对细胞进行体外转染来调节其行为。在这项研究中，我们证明了递送到豚鼠内耳或去细胞化耳蜗的 MSC 优先与肝素浓度高的区域结合。这为体外调节细胞行为提供了机会。我们评估了硫酸羧甲基葡聚糖（Cacicol），一种硫酸乙酰肝素类似物对螺旋神经节细胞和 MSC 的影响，证明了其对神经元存活的支持和对干细胞增殖的支持。
