Tokuyasu Naruo, Shomori Kohei, Amano Kuniki, Honjo Soichiro, Sakamoto Teruhisa, Watanabe Joji, Amisaki Masataka, Morimoto Masaki, Uchinaka Ei, Yagyu Takuki, Saito Hiroaki, Ito Hisao, Fujiwara Yoshiyuki
Division of Surgical Oncology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
†Division of Organ Pathology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
Yonago Acta Med. 2018 Jun 18;61(2):128-136. doi: 10.33160/yam.2018.06.005. eCollection 2018 Jun.
Indirubin, a constituent of the Chinese herbal medicine "Qing-Dai," has anti-cancer and anti-inflammatory activities. We aimed to evaluate the efficacy of indirubin for ameliorating colonic inflammation in a mouse model of inflammatory bowel disease.
Mice with dextran sulfate sodium (DSS)-induced acute and chronic colitis were treated with indirubin in their diet. Clinical and histologic changes were evaluated. In addition, colon levels of interleukin-6, a critical pro-inflammatory mediator, was detected by enzyme-linked immunosorbent assay.
In the model of acute colitis, indirubin treatment improved the loss of body weight. Histology of colonic tissue revealed that indirubin treatment improved the histology grading of colitis ( = 0.02), the extent of submucosal fibrosis ( = 0.018), the number of mucosal toluidine blue-positive cells ( = 0.004) and colon length ( = 0.01). In the model of chronic colitis, indirubin treatment had no significant effect on pathologic findings except for colon length ( = 0.003). However, indirubin administration significantly reduced colon levels of interleukin-6 in the chronic-colitis model ( = 0.001).
Our study clearly showed that oral intake of indirubin can improve murine DSS-induced colitis (which mimics human inflammatory bowel disease).
靛玉红是中药“青黛”的一种成分，具有抗癌和抗炎活性。我们旨在评估靛玉红在炎症性肠病小鼠模型中改善结肠炎症的疗效。
用葡聚糖硫酸钠（DSS）诱导急性和慢性结肠炎的小鼠在饮食中给予靛玉红治疗。评估临床和组织学变化。此外，通过酶联免疫吸附测定法检测结肠中关键促炎介质白细胞介素-6的水平。
在急性结肠炎模型中，靛玉红治疗改善了体重减轻。结肠组织学显示，靛玉红治疗改善了结肠炎的组织学分级（P = 0.02）、黏膜下纤维化程度（P = 0.018）、黏膜甲苯胺蓝阳性细胞数量（P = 0.004）和结肠长度（P = 0.01）。在慢性结肠炎模型中，靛玉红治疗除对结肠长度有影响外（P = 0.003），对病理结果无显著影响。然而，在慢性结肠炎模型中，给予靛玉红显著降低了结肠白细胞介素-6水平（P = 0.001）。
我们的研究清楚地表明，口服靛玉红可改善小鼠DSS诱导的结肠炎（模拟人类炎症性肠病）。
