Shewry P R, Bunce N A, Kreis M, Forde B G
Biochem Genet. 1985 Jun;23(5-6):391-404. doi: 10.1007/BF00499082.
The Hor 1 locus of barley encodes a group of seed storage polypeptides called C hordein. Two-dimensional electrophoretic analysis of C-hordein fractions from six cultivars with different alleles at the Hor 1 locus showed extensive polymorphism. A total of 34 major polypeptides was mapped, with between 4 and 18 present in each cultivar. There was less variation among the same cultivars in the numbers (6 to 10) of restriction fragments of genomic DNA which hybridized to a cDNA clone related to C hordein. The total number of restriction fragments was also lower (22), and most pairs of cultivars had more restriction fragments than polypeptides in common. A total number of about 20-30 C-hordein genes per haploid genome was estimated. The results indicate that cultivars differ mainly in the extent of gene and polypeptide divergence, rather than in the degree of gene reiteration. They are consistent with the proposed origin of the multiple structural genes at the Hor 1 locus by the duplication and divergence of a single ancestral gene.
大麦的Hor 1位点编码一组名为C-醇溶蛋白的种子贮藏多肽。对来自Hor 1位点具有不同等位基因的六个品种的C-醇溶蛋白组分进行二维电泳分析，结果显示出广泛的多态性。总共定位了34种主要多肽，每个品种中有4至18种。与C-醇溶蛋白相关的cDNA克隆杂交的基因组DNA限制性片段数量（6至10个）在相同品种之间变化较小。限制性片段总数也较低（22个），大多数品种对之间共同的限制性片段比多肽更多。估计每个单倍体基因组中约有20 - 30个C-醇溶蛋白基因。结果表明，品种之间的差异主要在于基因和多肽的分化程度，而非基因重复程度。这些结果与通过单个祖先基因的复制和分化在Hor 1位点产生多个结构基因的推测起源一致。
