大麦胚乳多聚腺苷酸（poly A+）RNA来源的cDNA序列的分子克隆与分析：B醇溶蛋白相关克隆的鉴定
Molecular cloning and analysis of cDNA sequences derived from poly A+ RNA from barley endosperm: identification of B hordein related clones.
作者信息
Forde B G, Kreis M, Bahramian M B, Matthews J A, Miflin B J, Thompson R D, Bartels D, Flavell R B
出版信息
Nucleic Acids Res. 1981 Dec 21;9(24):6689-707. doi: 10.1093/nar/9.24.6689.
A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.
利用来自大麦胚乳（品种为森丹斯）膜结合多核糖体的聚腺苷酸加尾RNA作为模板，在细菌质粒pPH207和pBR322中构建了130多个互补DNA（cDNA）克隆。通过交叉杂交分析和质粒选择的信使核糖核酸（mRNA）的体外翻译，鉴定出了54个B族大麦醇溶蛋白cDNA克隆。11个B族大麦醇溶蛋白cDNA克隆与大小分级RNA的Northern印迹杂交表明，B族大麦醇溶蛋白mRNA约为1300个核苷酸长。一个cDNA克隆pHvE-c16已进行了部分测序，通过与C末端和其他肽序列比较表明，它与B1族大麦醇溶蛋白多肽相关。对B族大麦醇溶蛋白cDNA克隆的分析结果支持了这样一种观点，即指定B族大麦醇溶蛋白的Hor 2基因座是复杂的，并且编码一组相关的mRNA种类。
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