Dangi Arun K, Sinha Rajeshwari, Dwivedi Shailja, Gupta Sanjeev K, Shukla Pratyoosh
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, India.
Independent Researcher, New Delhi, India.
Front Pharmacol. 2018 Jun 12;9:630. doi: 10.3389/fphar.2018.00630. eCollection 2018.
The present day modern formulation practices for drugs are based on newer tools and techniques toward effective utilization. The methods of antibody formulations are to be revolutionized based on techniques of cell engineering and gene editing. In the present review, we have discussed innovations in cell engineering toward production of novel antibodies for therapeutic applications. Moreover, this review deciphers the use of RNAi, ribozyme engineering, CRISPR-Cas-based techniques for better strategies for antibody production. Overall, this review describes the multidisciplinary aspects of the production of therapeutic proteins that has gained more attention due to its increasing demand.
当今药物的现代制剂实践基于更新的工具和技术以实现有效利用。抗体制剂的方法将基于细胞工程和基因编辑技术进行革新。在本综述中,我们讨论了细胞工程在生产用于治疗应用的新型抗体方面的创新。此外,本综述还解读了RNA干扰、核酶工程、基于CRISPR-Cas的技术在优化抗体生产策略方面的应用。总体而言,本综述描述了治疗性蛋白质生产的多学科方面,由于其需求不断增加,该领域已受到更多关注。