Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Penang, Minden, Malaysia.
Department of Biotechnology, Faculty of Applied Sciences, UCSI University, No 1 Jalan Menara Gading, UCSI Heights, Taman Connaught, 56000, Kuala Lumpur, Cheras, Malaysia.
Mol Biol Rep. 2022 Nov;49(11):10593-10608. doi: 10.1007/s11033-022-07651-3. Epub 2022 Jun 8.
Antibodies have proven to be remarkably successful for biomedical applications. They play important roles in epidemiology and medicine from diagnostics of diseases to therapeutics, treating diseases from incessant chronic diseases such as rheumatology to pandemic outbreaks. With no end in sight for the demand for antibody products, optimizations and new techniques must be expanded to accommodate this.
This review discusses optimizations and techniques for antibody production through choice of discovery platforms, expression systems, cell culture mediums, and other strategies to increase expression yield. Each system has its own merits and demerits, and the strategy chosen is critical in addressing various biological aspects.
There is still insufficient evidence to validate the efficacy of some of these techniques, and further research is needed to consolidate these industrial production systems. There is no doubt that more strategies, systems, and pipelines will contribute to enhance biopharmaceutical production.
抗体在生物医学应用中已被证明是非常成功的。它们在流行病学和医学中发挥着重要作用，从疾病的诊断到治疗，从风湿等持续的慢性疾病到流行病爆发，都有治疗作用。由于对抗体产品的需求没有尽头，因此必须扩大优化和新技术，以适应这一需求。
本文通过选择发现平台、表达系统、细胞培养基和其他提高表达产量的策略，讨论了抗体生产的优化和技术。每个系统都有其优点和缺点，所选择的策略对于解决各种生物学问题至关重要。
目前还没有足够的证据来验证其中一些技术的疗效，需要进一步的研究来整合这些工业生产系统。毫无疑问，更多的策略、系统和管道将有助于提高生物制药的生产。
