Carella Francesca, Aceto Serena, Mangoni Olga, Mollica Maria Pina, Cavaliere Gina, Trinchese Giovanna, Aniello Francesco, De Vico Gionata
Department of Biology, University of Naples Federico II, Naples, Italy.
Front Physiol. 2018 Jun 12;9:683. doi: 10.3389/fphys.2018.00683. eCollection 2018.
The bivalve has a broad geographic distribution, represent an important species for the ecology of coastal waters, also constituting a major aquaculture species. In the present work, molecular and tissue biomarkers were examined in mussel populations () located in four different areas of the coastal water of the Campania Region. During an annual life cycle, we analyzed the expression patterns of several genes commonly used to estimate cellular stress response and damage, namely , , , , and , related tissue lesions (pathogens, inflammations, digestive tubules damage), oxidative stress indicators (HO, SOD specific activity) and associated environmental data. The computed Principal Component Analysis showed that the areas were discernible based on the environmental data and biomarker results. About animal health status, mussels from Gulf of Pozzuoli and Naples's harbor did show a thinnest epithelial cell of digestive tubules compared to mussels sampled from other sampling sites; moreover, high prevalence of cases of intersex in three of the examinated areas were observed. The presence of a potential zoonotic pathogen () was identified, appearing as an important possible emerging disease. We also reported the OIE notifiable protozoa in three areas out of four. The likely impact of both observed pathogens on the mussel health and shellfish aquaculture needs to be urgently addressed. Results are discussed considering animal histopathological health parameters and biological effects.
双壳贝类具有广泛的地理分布，是沿海水域生态的重要物种，也是主要的水产养殖物种。在本研究中，我们对位于坎帕尼亚地区沿海水域四个不同区域的贻贝种群进行了分子和组织生物标志物检测。在一年的生命周期内，我们分析了几个常用于评估细胞应激反应和损伤的基因的表达模式，即 、 、 、 和 ，以及相关的组织损伤（病原体、炎症、消化小管损伤）、氧化应激指标（HO、SOD 比活性）和相关环境数据。计算得到的主成分分析表明，根据环境数据和生物标志物结果可以区分这些区域。关于动物健康状况，与从其他采样点采集的贻贝相比，波佐利湾和那不勒斯港的贻贝消化小管上皮细胞最薄；此外，在三个检测区域中观察到了较高比例的雌雄同体病例。鉴定出了一种潜在的人畜共患病原体（ ），这似乎是一种重要的可能出现的新兴疾病。我们还报告了在四个区域中的三个区域发现了国际兽疫局应通报的原生动物 。这两种观察到的病原体对贻贝健康和贝类养殖的可能影响亟待解决。我们结合动物组织病理学健康参数和生物学效应进行了结果讨论。
