Santoro Mario, Marchiori Erica, Palomba Marialetizia, Uberti Barbara Degli, Marcer Federica, Mattiucci Simonetta
Department of Integrative Marine Ecology, Stazione Zoologica Anthon Dohrn, Villa Comunale, 80121 Naples, Italy.
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 35020 Legnaro, Padova, Italy.
Pathogens. 2020 Feb 13;9(2):118. doi: 10.3390/pathogens9020118.
(Anisakidae), a pathogenic nematode of sea turtles, may cause ulcerous gastritis with different degrees of severity. Previous studies demonstrated a high prevalence of infection in the Mediterranean loggerhead turtle (), although no data on the potential intermediate hosts of this nematode has been published thus far from the Mediterranean basin. Here, using molecular analyses, we demonstrated that the cross sections of nematode larvae observed histologically in Mediterranean mussels () collected from a farm along the Tyrrhenian coast of southern Italy belong to . The BLAST analysis of sequences at the ITS2 region of rDNA and mtDNA gene loci here obtained from samples of two Mediterranean mussels containing nematode larvae showed 100% homology with those at the same gene loci from the adults of collected from the Mediterranean Sea and deposited in GenBank. To our knowledge, this study is the first to present data on a potential intermediate host of in the Mediterranean basin and to report a nematode parasite from the Mediterranean mussel.
异尖科（Anisakidae）是海龟的一种致病性线虫，可引起不同严重程度的溃疡性胃炎。先前的研究表明，地中海蠵龟（Caretta caretta）的感染率很高，尽管迄今为止地中海盆地尚未发表关于这种线虫潜在中间宿主的数据。在这里，通过分子分析，我们证明，从意大利南部第勒尼安海岸一个养殖场采集的地中海贻贝（Mytilus galloprovincialis）组织学观察到的线虫幼虫横切面属于异尖线虫（Anisakis simplex）。从含有线虫幼虫的两只地中海贻贝样本中获得的rDNA ITS2区域和mtDNA cox1基因位点序列的BLAST分析显示，与从地中海采集并保存在GenBank中的异尖线虫成虫相同基因位点的序列具有100%的同源性。据我们所知，本研究首次提供了地中海盆地异尖线虫潜在中间宿主的数据，并报告了来自地中海贻贝的一种线虫寄生虫。
