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胞外多糖生物合成:一种改善苹果酸-乳酸发酵起始菌性能的工具

Exopolysaccharide Biosynthesis, a Tool to Improve Malolactic Starter Performance.

作者信息

Dimopoulou Maria, Raffenne Jerôme, Claisse Olivier, Miot-Sertier Cécile, Iturmendi Nerea, Moine Virginie, Coulon Joana, Dols-Lafargue Marguerite

机构信息

Université de Bordeaux, EA 4577 Œnologie, INRA, USC 1366, ISVV, Bordeaux INP, Villenave-d'Ornon, France.

Biolaffort, Bordeaux, France.

出版信息

Front Microbiol. 2018 Jun 12;9:1276. doi: 10.3389/fmicb.2018.01276. eCollection 2018.

Abstract

is the lactic acid bacterium that most commonly drives malolactic fermentation (MLF) in wine. Though the importance of MLF in terms of wine microbial stability and sensory improvement is well established, it remains a winemaking step not so easy to control. displays many adaptation tools to resist the harsh wine conditions which explain its natural dominance at this stage of winemaking. Previous findings showed that capsular polysaccharides and endogenous produced dextran increased the survival rate and the conservation time of malolactic starters. In this paper, we showed that exopolysaccharides specific production rates were increased in the presence of single stressors relevant to wine (pH, ethanol). The transcription of the associated genes was investigated in distinct strains. The conditions in which eps genes and EPS synthesis were most stimulated were then evaluated for the production of freeze dried malolactic starters, for acclimation procedures and for MLF efficiency. Sensory analysis tests on the resulting wines were finally performed.

摘要

是最常用于驱动葡萄酒中苹果酸-乳酸发酵(MLF)的乳酸菌。尽管MLF在葡萄酒微生物稳定性和感官改善方面的重要性已得到充分确立,但它仍是葡萄酒酿造过程中一个不太容易控制的步骤。 展示了许多适应工具来抵抗恶劣的葡萄酒环境,这解释了它在葡萄酒酿造这一阶段的自然优势。先前的研究结果表明,荚膜多糖和内源性产生的葡聚糖提高了苹果酸-乳酸发酵起始菌的存活率和保存时间。在本文中,我们表明,在与葡萄酒相关的单一应激源(pH值、乙醇)存在的情况下,胞外多糖的特定生产率会增加。在不同菌株中研究了相关基因的转录情况。然后评估了最能刺激eps基因和EPS合成的条件,用于冻干苹果酸-乳酸发酵起始菌的生产、驯化程序和MLF效率。最后对所得葡萄酒进行了感官分析测试。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9ad/6006919/0bb43ec64faf/fmicb-09-01276-g0001.jpg

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