Choca J I, Kwatra M M, Hosey M M, Green R D
Biochem Biophys Res Commun. 1985 Aug 30;131(1):115-21. doi: 10.1016/0006-291x(85)91778-4.
N6(L-phenylisopropyl)adenosine (L-PIA) and N6(3-iodo-4-azido benzyl)-adenosine (IAzBA) inhibit the adenylate cyclase activity in synaptic membranes of chick cerebellum via Ri adenosine receptors. [3H]L-PIA and [125I]AzBA bind to these membranes with Kd values of approximately 1 nM and Bmax values of approximately 1000 fmol/mg protein. Photolysis of [125I]AzBA bound to synaptic membranes results in the specific incorporation of radioactivity into a protein with Mr = 36,000. This photoincorporation is blocked by simultaneous exposure to L-PIA, theophylline, an adenosine receptor antagonist, or Gpp(NH)p, but not by cytosine, suggesting that the 36,000 dalton protein is the Ri adenosine receptor or a subunit of the receptor that contains the adenosine binding site.
N6-(L-苯异丙基)腺苷（L-PIA）和N6-(3-碘-4-叠氮苄基)腺苷（IAzBA）通过Ri型腺苷受体抑制鸡小脑突触膜中的腺苷酸环化酶活性。[3H]L-PIA和[125I]AzBA与这些膜结合，其解离常数（Kd）值约为1 nM，最大结合量（Bmax）值约为1000 fmol/mg蛋白质。与突触膜结合的[125I]AzBA经光解后，放射性特异性掺入一种分子量为36,000的蛋白质中。同时暴露于L-PIA、茶碱（一种腺苷受体拮抗剂）或鸟苷-5'-O-(3-硫代三磷酸)（Gpp(NH)p）可阻断这种光掺入，但胞嘧啶不能阻断，这表明36,000道尔顿的蛋白质是Ri型腺苷受体或包含腺苷结合位点的受体亚基。
